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<p>Although microparticle-enhanced cultivation (MPEC) significantly improves transglutaminase (TGase) yield, its underlying mechanism remains unclear. This study elucidated how talc and CaCO<sub>3</sub> boost TGase production in <i>Streptomyces mobaraensis</i> HVCP-Sm1. Transcriptomic analysis revealed that both microparticles reprogram energy metabolism, but through distinct pathways: talc primarily enhanced energy conversion efficiency by activating ATP synthase and proton transmembrane transport, while CaCO<sub>3</sub> augmented the supply of ATP and the substrate glutamine. Furthermore, overexpression of XDH-D (Gene 5232), encoding xanthine dehydrogenase subunit D, increased TGase titer by 39.4% to 21.24 U/mL. These findings demonstrate that talc and CaCO<sub>3</sub> promote TGase synthesis by differentially rewiring central energy metabolism, and highlight the critical role of energy metabolic regulation in <i>Streptomyces</i> secondary metabolism.</p>

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Energy metabolism reprogramming in Streptomyces mobaraensis driven by microparticles enhances transglutaminase production

  • 期刊论文

  • 发表日期：

    2026年4月1日

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  • Tingting Chang,
  • Luyao Bian,
  • Chong Zhang

摘要

Although microparticle-enhanced cultivation (MPEC) significantly improves transglutaminase (TGase) yield, its underlying mechanism remains unclear. This study elucidated how talc and CaCO3 boost TGase production in Streptomyces mobaraensis HVCP-Sm1. Transcriptomic analysis revealed that both microparticles reprogram energy metabolism, but through distinct pathways: talc primarily enhanced energy conversion efficiency by activating ATP synthase and proton transmembrane transport, while CaCO3 augmented the supply of ATP and the substrate glutamine. Furthermore, overexpression of XDH-D (Gene 5232), encoding xanthine dehydrogenase subunit D, increased TGase titer by 39.4% to 21.24 U/mL. These findings demonstrate that talc and CaCO3 promote TGase synthesis by differentially rewiring central energy metabolism, and highlight the critical role of energy metabolic regulation in Streptomyces secondary metabolism.