Scholarlink

<p>The emergence and persistence of plasmid-mediated polymyxin resistance in Brazilian poultry production pose a significant One Health challenge. Here, cloacal swabs from 202 broilers across four farms in the State of Rio de Janeiro yielded 125 <i>Enterobacteriaceae</i> isolates growing on polymyxin-EMB agar. <i>Escherichia coli</i> accounted for 99% of resistant isolates, with one <i>Klebsiella pneumoniae</i>. Multidrug resistance (MDR) was observed in 75% of polymyxin-resistant strains. PCR screening revealed <i>mcr-1</i> and <i>mcr-5</i> genes. Conjugation assays demonstrated horizontal transfer of <i>mcr-1</i> plasmids (48.5–194&#xa0;kb). MLST assigned key strains to ST10 and ST48, both within the high-risk CC10 lineage. These findings underscore the entrenched nature of polymyxin resistance despite regulatory bans, highlight the risk of zoonotic transmission of MDR determinants, and call for enhanced surveillance, biosecurity and alternative interventions to mitigate the spread of mobile polymyxin resistance in poultry environments.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Detection of polymyxin-resistant Enterobacteriaceae from poultry farms in Brazil: continued mcr gene dissemination

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月18日

查看全文
  • Bruno Rocha Pribul,
  • Kathelyn Soares dos Santos,
  • Ramon Pimenta,
  • Orlando Carlos da Conceição-Neto,
  • Ana Paula D’Alincourt Carvalho-Assef,
  • Miliane Moreira Soares de Souza,
  • Cláudio Marcos Rocha-de-Souza

摘要

The emergence and persistence of plasmid-mediated polymyxin resistance in Brazilian poultry production pose a significant One Health challenge. Here, cloacal swabs from 202 broilers across four farms in the State of Rio de Janeiro yielded 125 Enterobacteriaceae isolates growing on polymyxin-EMB agar. Escherichia coli accounted for 99% of resistant isolates, with one Klebsiella pneumoniae. Multidrug resistance (MDR) was observed in 75% of polymyxin-resistant strains. PCR screening revealed mcr-1 and mcr-5 genes. Conjugation assays demonstrated horizontal transfer of mcr-1 plasmids (48.5–194 kb). MLST assigned key strains to ST10 and ST48, both within the high-risk CC10 lineage. These findings underscore the entrenched nature of polymyxin resistance despite regulatory bans, highlight the risk of zoonotic transmission of MDR determinants, and call for enhanced surveillance, biosecurity and alternative interventions to mitigate the spread of mobile polymyxin resistance in poultry environments.