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<p>This study aimed to reveal the landfill bacterium <i>Priestia</i> sp. isolate TL1 as a PHA-producing bacterium via PHA quantification and gene annotation studies. Screening of landfill isolates identified <i>Priestia</i> sp. isolate TL1 as a promising PHA producer. Evaluation on the effect of different glucose concentrations (1–5%) on PHA accumulation revealed that optimal production was observed at 3% of glucose concentration, while excessive glucose levels negatively affected PHA yield. Statistical analysis indicated no significant association between cell dry weight and PHA yield (<i>p</i> &gt; 0.05), suggesting that biomass accumulation does not directly predict PHA production efficiency. Whole-genome sequencing performed on isolate TL1 confirmed the taxonomic identity of TL1 as <i>Priestia</i> sp. Gene annotation identified key genes associated with PHA biosynthesis, which included <i>phaC</i>, encoding a Class IV PHA synthase. This suggested that PHA production by isolate TL1 may be restricted to short-chain-length PHAs. These findings highlight the potential of <i>Priestia</i> sp. (isolate TL1) for cost-effective PHA production for sustainable bioplastic development.</p>

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Polyhydroxyalkanoate (PHA) production by landfill isolate Priestia sp. revealed by preparatory glucose-induced tests and genome-annotations

  • 期刊论文

  • 发表日期：

    2025年12月23日

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  • Nena Yokoyama,
  • Clariss Hui Peng Goh,
  • Chun Yuan Tan,
  • Wei Yee Wee,
  • Adeline Su Yien Ting

摘要

This study aimed to reveal the landfill bacterium Priestia sp. isolate TL1 as a PHA-producing bacterium via PHA quantification and gene annotation studies. Screening of landfill isolates identified Priestia sp. isolate TL1 as a promising PHA producer. Evaluation on the effect of different glucose concentrations (1–5%) on PHA accumulation revealed that optimal production was observed at 3% of glucose concentration, while excessive glucose levels negatively affected PHA yield. Statistical analysis indicated no significant association between cell dry weight and PHA yield (p > 0.05), suggesting that biomass accumulation does not directly predict PHA production efficiency. Whole-genome sequencing performed on isolate TL1 confirmed the taxonomic identity of TL1 as Priestia sp. Gene annotation identified key genes associated with PHA biosynthesis, which included phaC, encoding a Class IV PHA synthase. This suggested that PHA production by isolate TL1 may be restricted to short-chain-length PHAs. These findings highlight the potential of Priestia sp. (isolate TL1) for cost-effective PHA production for sustainable bioplastic development.