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<p>Early diagnosis of sporotrichosis, along with the surveillance of fungal circulation in both human and domestic animal populations, is critical for global health. Although the gold standard method is microbiological culture followed by microscopic analysis of the fungus, this methodology requires a long period and skilled professionals for pathogen identification. We developed a molecular assay and conducted a preliminary evaluation using clinical samples using Loop-Mediated Isothermal Amplification (LAMP) technology that detects <i>Sporothrix schenckii</i> and <i>Sporothrix brasiliensis</i> fungi, with results in 30&#xa0;min. Results are visually interpreted and do not require DNA extraction, making it a promising tool for point-of-care diagnosis in veterinary settings.</p>

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Veterinary application of LAMP: a simple and visual detection tool for feline sporotrichosis

  • 期刊论文

  • 发表日期：

    2025年5月28日

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  • Lívia do Carmo Silva,
  • Isabela Cristina de Oliveira dos Santos,
  • Juliana Santana de Curcio,
  • Allana de Paula Castilho,
  • João Vitor Gregolan Barbosa,
  • Luiz Henrique Alves Costa,
  • Álvaro Ferreira Júnior,
  • Elisângela de Paula Silveira-Lacerda

摘要

Early diagnosis of sporotrichosis, along with the surveillance of fungal circulation in both human and domestic animal populations, is critical for global health. Although the gold standard method is microbiological culture followed by microscopic analysis of the fungus, this methodology requires a long period and skilled professionals for pathogen identification. We developed a molecular assay and conducted a preliminary evaluation using clinical samples using Loop-Mediated Isothermal Amplification (LAMP) technology that detects Sporothrix schenckii and Sporothrix brasiliensis fungi, with results in 30 min. Results are visually interpreted and do not require DNA extraction, making it a promising tool for point-of-care diagnosis in veterinary settings.