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<p>This study aimed to evaluate the in vitro antiviral activity of ethanolic extracts from <i>Azadirachta indica</i> and <i>Melia azedarach</i> during the replication cycle of the CAEV-Cork and MVV-K1514 strains, both part of the small ruminant lentivirus (SRLV) group, in ovine third eyelid (OTE) cells. The crude extracts (CE), along with the ethyl acetate (EAF) and methanol (MF) organic fractions from these plants, were tested in treatments applied before, during, and after viral inoculation of the CAEV-Cork and MVV-K1514 strains in separate OTE cell cultures. Following these treatments, the most effective results were subjected to viral titration analysis. The findings indicate that the EAF of <i>A. indica</i> and <i>M. azedarach</i> may have reduced the viral titer of the CAEV-Cork strain by 316-fold after inoculation and by 1.995-fold when applied concomitantly with inoculation, respectively. For the MVV-K1514 strain, the EAF of <i>A. indica</i> achieved a 1,000-fold inhibition when applied simultaneously with viral inoculation. These results suggest that extracts from <i>Meliaceae</i> plants influenced all phases of the SRLV replication cycle, significantly inhibiting the activity of both viral strains, although complete viral elimination was not achieved.</p>

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Antiviral activity of Azadirachta indica A. Juss and Melia azedarach extracts during the replication cycle of small ruminant lentiviruses

  • 期刊论文

  • 发表日期：

    2025年4月26日

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  • Ana Lídia Madeira de Sousa,
  • Raymundo Rizaldo Pinheiro,
  • Juscilânia Furtado Araújo,
  • Renato Mesquita Peixoto,
  • Ana Milena César Lima,
  • Selene Maia de Morais,
  • Samara Cristina Rocha Souza,
  • Gabriel Paula Amaral,
  • Maria Fátima da Silva Teixeira,
  • Alice Andrioli

摘要

This study aimed to evaluate the in vitro antiviral activity of ethanolic extracts from Azadirachta indica and Melia azedarach during the replication cycle of the CAEV-Cork and MVV-K1514 strains, both part of the small ruminant lentivirus (SRLV) group, in ovine third eyelid (OTE) cells. The crude extracts (CE), along with the ethyl acetate (EAF) and methanol (MF) organic fractions from these plants, were tested in treatments applied before, during, and after viral inoculation of the CAEV-Cork and MVV-K1514 strains in separate OTE cell cultures. Following these treatments, the most effective results were subjected to viral titration analysis. The findings indicate that the EAF of A. indica and M. azedarach may have reduced the viral titer of the CAEV-Cork strain by 316-fold after inoculation and by 1.995-fold when applied concomitantly with inoculation, respectively. For the MVV-K1514 strain, the EAF of A. indica achieved a 1,000-fold inhibition when applied simultaneously with viral inoculation. These results suggest that extracts from Meliaceae plants influenced all phases of the SRLV replication cycle, significantly inhibiting the activity of both viral strains, although complete viral elimination was not achieved.