<p>Infection with hepatitis B virus (HBV) is a global health problem, causing diseases such as acute and chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Numerous studies in the literature have demonstrated hepatitis B surface antigen (HBsAg) as the first virological marker of HBV infection, and that serum HBsAg levels are reported to be an important criterion for the diagnosis and management of HBV infection. Therefore, the sensitive and selective detection of HBsAg is critical for the early diagnosis of hepatitis B and for the management of the diseases associated with HBV infection. Electrochemical immunosensors play an important role in the early diagnosis of infection with hepatitis B virus since they are rapid, easy-to-use, and reliable diagnostic tools with a possibility of on-site detection. Besides, they might have superior performance in terms of multiple analytical parameters compared to more commonly used alternatives in the clinic, including ELISA. Herein, we review the reported success and potential of electrochemical biosensors for the detection of HBsAg in the early diagnosis and monitoring of hepatitis B virus infection, and provide a brief overview for future studies, with the ultimate aim of directing more research focus into this topic.</p>