Scholarlink

<p>This study aims to explore saline-alkaline soil remediation by cultivating wheat inoculated with local bacteria (<i>Bacillus tropicus</i> N5) and arbuscular mycorrhizal fungi (AMF). It emphasizes elucidating bacterial-fungal interactions and wheat’s adaptive responses to environmental variations in saline-alkaline soils. (1) Isolation and characterization of halotolerant bacteria from saline-alkaline soils; (2) in vitro assessment of the plant growth-promoting traits of the selected strains; (3) optimization of inoculation dosage through soil saturation experiments; and (4) evaluation of remediation performance via pot experiments, during which the interaction mechanisms among salt-tolerant bacteria, AMF, and wheat were analyzed by measuring key ion contents (e.g., Na+, K+) in plants and the soil bacterial community structure. After inoculating wheat with AMF and B. <i>tropicus</i> N5, the physical and chemical properties of the saline-alkaline soil improved significantly. N5 enhanced the infection rate of AMF by 30.6%, and both N5 and AMF synergistically promoted biomass accumulation and nutrient content in wheat roots, stems, and leaves. Specifically, the Na+/K+ ratio in wheat roots, stems, and leaves decreased by 42.82%, 50.70%, and 42.52%, respectively, indicating an enhanced tolerance of wheat to saline-alkaline stress. The diversity of rhizosphere microbial communities increased markedly, and several beneficial functional microorganisms were significantly enriched. Correlation analysis revealed a strong positive association among wheat biomass, rhizosphere microbial diversity, and soil remediation efficiency. Wheat inoculated with AMF and B. <i>tropicus</i> N5 substantially enhanced soil functional microorganisms and related genes, likely due to the synergistic effects of AMF’s hyphal networks, N5’s growth-promoting traits, and wheat’s carbon input through root exudates. These results provide theoretical support for employing native salt-tolerant microorganisms and AMF to improve wheat productivity and rehabilitate the capacity of saline-alkaline soils.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Synergistic Remediation of Saline-alkali Soil Through Inoculation of Wheat with Arbuscular Mycorrhizal Fungi and Bacillus Tropicus N5

  • 期刊论文

  • 发表日期：

    2026年2月10日

查看全文
  • Meng-meng Zhao,
  • Jingwen Zhang,
  • Qi An,
  • Wanqing Ma,
  • Mingxia Zhang,
  • Zixuan Guo,
  • Xiong Chen,
  • Fanfan Yang,
  • Liang Dai

摘要

This study aims to explore saline-alkaline soil remediation by cultivating wheat inoculated with local bacteria (Bacillus tropicus N5) and arbuscular mycorrhizal fungi (AMF). It emphasizes elucidating bacterial-fungal interactions and wheat’s adaptive responses to environmental variations in saline-alkaline soils. (1) Isolation and characterization of halotolerant bacteria from saline-alkaline soils; (2) in vitro assessment of the plant growth-promoting traits of the selected strains; (3) optimization of inoculation dosage through soil saturation experiments; and (4) evaluation of remediation performance via pot experiments, during which the interaction mechanisms among salt-tolerant bacteria, AMF, and wheat were analyzed by measuring key ion contents (e.g., Na+, K+) in plants and the soil bacterial community structure. After inoculating wheat with AMF and B. tropicus N5, the physical and chemical properties of the saline-alkaline soil improved significantly. N5 enhanced the infection rate of AMF by 30.6%, and both N5 and AMF synergistically promoted biomass accumulation and nutrient content in wheat roots, stems, and leaves. Specifically, the Na+/K+ ratio in wheat roots, stems, and leaves decreased by 42.82%, 50.70%, and 42.52%, respectively, indicating an enhanced tolerance of wheat to saline-alkaline stress. The diversity of rhizosphere microbial communities increased markedly, and several beneficial functional microorganisms were significantly enriched. Correlation analysis revealed a strong positive association among wheat biomass, rhizosphere microbial diversity, and soil remediation efficiency. Wheat inoculated with AMF and B. tropicus N5 substantially enhanced soil functional microorganisms and related genes, likely due to the synergistic effects of AMF’s hyphal networks, N5’s growth-promoting traits, and wheat’s carbon input through root exudates. These results provide theoretical support for employing native salt-tolerant microorganisms and AMF to improve wheat productivity and rehabilitate the capacity of saline-alkaline soils.

Graphical Abstract