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<p>Mangroves support coastal protection and ecosystem stability. This study investigates the diversity and function of rhizobacteria in the Bhitarkanika mangroves, Odisha, employing both culture-independent and culture-dependent methods. The aim is to assess the role of bacteria in enhancing mangrove growth and their potential application in mangrove reforestation. Amplicon-based 16S rRNA gene sequencing served as a metagenomics tool to assess the structural and functional diversity of mangrove rhizobacteria. Additionally, culture-dependent techniques were employed to isolate and characterize biofilm-forming plant growth-promoting (PGP) bacteria (PGPB) from mangrove plants. Pot experiments in greenhouse conditions were conducted to assess the effects of PGPB inoculation on the growth and development of <i>Avicennia officinalis</i> L.. Culture-independent 16S rRNA amplicon (V3–V4) sequencing identified 1372 operational taxonomic units (OTUs), with Proteobacteria and Actinobacteria as dominant phyla, and predicted 408 crucial metabolic pathways. Culture-dependent studies yielded 738 bacterial isolates, among which six strains, <i>Pseudomonas aeruginosa</i> GAT2208B, <i>Enterobacter hormaechei</i> GAT2209B, <i>P. oryzihabitans</i> GAT2212B, <i>Serratia marcescens</i> GAT2216B, <i>P. otitidis</i> GAH2213B, and <i>Pantoea dispersa</i> GBH2203B, exhibited multiple PGP traits. These isolates significantly (<i>p</i> &lt; 0.05) enhanced seedling vigour (up to 248.43%) and plant growth such as root length (up to 63.32%), shoot length (up to 153.2%), dry weight (up to 588.73%), fresh weight (up to 557.89%), and photosynthetic pigment content by colonizing on the root surface of <i>A. officinalis</i> under greenhouse conditions. Among these, <i>P. aeruginosa</i> GAT2208B showed the highest potential. This study highlights the potential of mangrove-associated PGPB in boosting seedling growth and resilience, supporting their use in mangrove reforestation and ecosystem restoration.</p>

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Rhizospheric Bacterial Diversity and Biofilm-forming Plant Growth-promoting Bacteria from Bhitarkanika Mangrove, Odisha, India, for their Effects on the Growth of Avicennia officinalis L.

  • 期刊论文

  • 发表日期：

    2026年3月3日

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  • Sourav Kumar Panda,
  • Surajit Das

摘要

Mangroves support coastal protection and ecosystem stability. This study investigates the diversity and function of rhizobacteria in the Bhitarkanika mangroves, Odisha, employing both culture-independent and culture-dependent methods. The aim is to assess the role of bacteria in enhancing mangrove growth and their potential application in mangrove reforestation. Amplicon-based 16S rRNA gene sequencing served as a metagenomics tool to assess the structural and functional diversity of mangrove rhizobacteria. Additionally, culture-dependent techniques were employed to isolate and characterize biofilm-forming plant growth-promoting (PGP) bacteria (PGPB) from mangrove plants. Pot experiments in greenhouse conditions were conducted to assess the effects of PGPB inoculation on the growth and development of Avicennia officinalis L.. Culture-independent 16S rRNA amplicon (V3–V4) sequencing identified 1372 operational taxonomic units (OTUs), with Proteobacteria and Actinobacteria as dominant phyla, and predicted 408 crucial metabolic pathways. Culture-dependent studies yielded 738 bacterial isolates, among which six strains, Pseudomonas aeruginosa GAT2208B, Enterobacter hormaechei GAT2209B, P. oryzihabitans GAT2212B, Serratia marcescens GAT2216B, P. otitidis GAH2213B, and Pantoea dispersa GBH2203B, exhibited multiple PGP traits. These isolates significantly (p < 0.05) enhanced seedling vigour (up to 248.43%) and plant growth such as root length (up to 63.32%), shoot length (up to 153.2%), dry weight (up to 588.73%), fresh weight (up to 557.89%), and photosynthetic pigment content by colonizing on the root surface of A. officinalis under greenhouse conditions. Among these, P. aeruginosa GAT2208B showed the highest potential. This study highlights the potential of mangrove-associated PGPB in boosting seedling growth and resilience, supporting their use in mangrove reforestation and ecosystem restoration.