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<p>Purpose: Long-term nitrogen (N) and phosphorus (P) deposition may alter soil ecological stoichiometry, thus affecting soil microbial activity. Absolute and specific enzyme activities (normalized by microbial biomass carbon) refer to the microbial metabolic status or enzyme efficiency. Methods:&#xa0;A 10-year N- and/or P -addition experiment was conducted in a tropical secondary forest since 2009. The application rates were set as no addition (CK), 100&#xa0;kg N yr<sup>− 1</sup>ha<sup>− 1</sup> (N addition), 100&#xa0;kg P yr<sup>− 1</sup> ha<sup>− 1</sup> (P addition), 100&#xa0;kg N yr<sup>− 1</sup>ha<sup>− 1</sup>, and 100&#xa0;kg P yr<sup>− 1</sup>ha<sup>− 1</sup> (NP addition). We determined the long-term effects of N and/or P addition on the soil microbial biomass carbon, and the absolute and specific activities of β-1,4-glucosidase (BG), β-D-1,4-cellubiosidase (CB), β-1,4-N-acetyl-glucosaminidase (NAG), and acid phosphatase (ACP). Results:&#xa0;P and NP addition significantly increased the soil P content and reduced the soil C: P and N: P ratios. The absolute activity and specific enzyme activity of ACP decreased with P and NP addition but did not increase with N addition. P addition reduced the absolute and specific enzyme activity of NAG because of insufficient N supply compared with N addition. Conclusions:&#xa0;Our findings revealed that the P addition relieved microbial P limitation and increased microbial N demand, whereas the N deposition did not exacerbate microbial P demand. Future studies should track changes in the soil microbial community composition and functional genes expression better understand the long-term effects of N and P deposition on soil microbe and nutrient cycles.</p>

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Long-term Nitrogen Addition Did Not Aggravate Soil Microbial Phosphorus Demand in a Tropical Forest

  • 期刊论文

  • 发表日期：

    2026年1月20日

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  • Xuehua Hu,
  • Yubiao Lin,
  • Qifeng Mo,
  • Yingxu Fan,
  • Faming Wang,
  • Xiangping Tan,
  • Qian Zhao

摘要

Purpose: Long-term nitrogen (N) and phosphorus (P) deposition may alter soil ecological stoichiometry, thus affecting soil microbial activity. Absolute and specific enzyme activities (normalized by microbial biomass carbon) refer to the microbial metabolic status or enzyme efficiency. Methods: A 10-year N- and/or P -addition experiment was conducted in a tropical secondary forest since 2009. The application rates were set as no addition (CK), 100 kg N yr− 1ha− 1 (N addition), 100 kg P yr− 1 ha− 1 (P addition), 100 kg N yr− 1ha− 1, and 100 kg P yr− 1ha− 1 (NP addition). We determined the long-term effects of N and/or P addition on the soil microbial biomass carbon, and the absolute and specific activities of β-1,4-glucosidase (BG), β-D-1,4-cellubiosidase (CB), β-1,4-N-acetyl-glucosaminidase (NAG), and acid phosphatase (ACP). Results: P and NP addition significantly increased the soil P content and reduced the soil C: P and N: P ratios. The absolute activity and specific enzyme activity of ACP decreased with P and NP addition but did not increase with N addition. P addition reduced the absolute and specific enzyme activity of NAG because of insufficient N supply compared with N addition. Conclusions: Our findings revealed that the P addition relieved microbial P limitation and increased microbial N demand, whereas the N deposition did not exacerbate microbial P demand. Future studies should track changes in the soil microbial community composition and functional genes expression better understand the long-term effects of N and P deposition on soil microbe and nutrient cycles.