<p>This study aimed to evaluate the effectiveness of different feedstocks and their biochar as a climate-smart agricultural practice by examining its impact on CO₂, N₂O, CH₄ fluxes and global warming potential under incubation conditions.&#xa0;A laboratory incubation study was conducted to evaluate the effects of farmyard manure (FYM), maize straw (MS) and their respective biochar (BC) amendments on greenhouse gas emissions (GHG) and soil biochemical properties under controlled conditions. Soil CO₂, N₂O, and CH₄ emissions measured using gas chromatography at regular intervals throughout the incubation period. The priming effects of biochar on native soil organic carbon (SOC) mineralization were quantified using ¹³C isotope tracer techniques.&#xa0;Overall, BC additions notably increased soil pH, electrical conductivity, cation exchange capacity (CEC), NO₃-N, NH₄-N, P and K compared to control group or BC’s feedstocks (MS and FYM). Similarly, soil microbial biomass carbon (13.98%), microbial biomass nitrogen (17.76%), microbial biomass phosphorus (20.08%), soil organic carbon (43.56%), dissolved organic carbon (76.47%) and C: N (36.49) ratio also showed great result under FYMB40. Moreover, applications of BC including FYMB20,40 and MSB20,40 notably reduced GHG emissions fluxes. For instance, FYMB40 mitigated cumulative CO₂, N₂O and CH₄ emissions up to 28.11, 23.83 and 42.63% respectively. Besides this, a significant decline also observed in global warming potential (GWP) under FYMB40 (43.81%) and MSB40 (42.40%) against control group. BC addition also had a negative priming effect on native SOC mineralization which was attributed to the CO₂ emission reduction. Higher doses of BC including FYMB40 and MSB40 had the most prominent effect on soil GHG emissions mitigation, followed by FMB20 and MSB20.&#xa0;Overall, the results of study suggest that BC addition had a remarkable potential on soil GHG emissions mitigations, however, its impact varies according to BC feedstock types and application rates.</p>