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Purpose <p>In recent decades, the widespread and indiscriminate use of pesticides has led to numerous adverse effects, including declines in biological diversity. This study aimed to investigate the effects of glyphosate on soil microbial communities.</p> Methods <p>Internal Transcribed Spacer (ITS) metabarcoding was employed to assess fungal community structure. This study examined the diversity of crop soil (CS), soil samples enriched with glyphosate (ES), and rhizosphere soil (RS). All samples were collected from an agricultural area contaminated with glyphosate in the Araucária municipality, Paraná State, Brazil. Principal coordinates analysis (PCoA) was used to identify variations in community structure.</p> Results <p>The results revealed that glyphosate enrichment altered the structure of the fungal community, with a reduction in the relative abundance of genera such as <i>Conocybe</i>, <i>Vermispora</i>, and <i>Setophoma</i>. In contrast, <i>Lophiostoma</i>, <i>Spizellomyces</i>, and <i>Saitozyma</i> showed increased relative abundances in the ES. The genera <i>Clonostachys</i> and <i>Fusarium</i> were exclusive to the soybean rhizosphere (RS). Additionally, PCoA revealed a similarity between CS and ES, whereas RS exhibited a distinct community composition.</p> Conclusion <p>This study demonstrated that glyphosate influences the composition of soil fungal communities by either suppressing or favoring specific taxa. Some of these taxa may have bioremediation potential and warrant further investigations. Moreover, metabarcoding data from RS suggests that the presence of soybeans significantly influences microbial community structure, highlighting their crucial role in pollutant degradation, soil functionality, and overall soil health.</p>

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Metabarcoding Analysis of Fungal Communities in Glyphosate-Contaminated Soils

  • 期刊论文

  • 发表日期：

    2025年9月16日

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  • Marlon Roger Geraldo,
  • Franciele Baja,
  • Maria Eduarda Grisolia,
  • Flavia de Fatima Costa,
  • Emanuel Razzolini,
  • Gabriela Xavier Schneider,
  • Géssica Slompo de Deus,
  • Andreia Bueno da Silva,
  • Victor Patrial de Oliveira Vardanega,
  • Kassiely Zamarchi,
  • Ani Beatriz Jackisch Matsuura,
  • Ormezinda Celeste Cristo Fernandes,
  • Vania Aparecida Vicente,
  • Renata Rodrigues Gomes

摘要

Purpose

In recent decades, the widespread and indiscriminate use of pesticides has led to numerous adverse effects, including declines in biological diversity. This study aimed to investigate the effects of glyphosate on soil microbial communities.

Methods

Internal Transcribed Spacer (ITS) metabarcoding was employed to assess fungal community structure. This study examined the diversity of crop soil (CS), soil samples enriched with glyphosate (ES), and rhizosphere soil (RS). All samples were collected from an agricultural area contaminated with glyphosate in the Araucária municipality, Paraná State, Brazil. Principal coordinates analysis (PCoA) was used to identify variations in community structure.

Results

The results revealed that glyphosate enrichment altered the structure of the fungal community, with a reduction in the relative abundance of genera such as Conocybe, Vermispora, and Setophoma. In contrast, Lophiostoma, Spizellomyces, and Saitozyma showed increased relative abundances in the ES. The genera Clonostachys and Fusarium were exclusive to the soybean rhizosphere (RS). Additionally, PCoA revealed a similarity between CS and ES, whereas RS exhibited a distinct community composition.

Conclusion

This study demonstrated that glyphosate influences the composition of soil fungal communities by either suppressing or favoring specific taxa. Some of these taxa may have bioremediation potential and warrant further investigations. Moreover, metabarcoding data from RS suggests that the presence of soybeans significantly influences microbial community structure, highlighting their crucial role in pollutant degradation, soil functionality, and overall soil health.