<p>Zinc (Zn) is an essential micronutrient for both plant growth and human health. However, widespread zinc deficiency in soils has led to insufficient dietary intake in many regions, contributing to various health issues. This review provides a comprehensive overview of zinc biofortification, focusing on the status of zinc in soil, plants, and its subsequent impact on human nutrition. We delve into the intricate mechanisms of zinc transporters governing the uptake, translocation, and accumulation of zinc in plants, shedding light on the crucial pathways influencing zinc bioavailability. Furthermore, this review explores various biofortification strategies aimed at enhancing zinc content in crops. Agronomical approaches, such as nutrient management and soil amendments, are discussed alongside genetic interventions, including traditional breeding methods and modern genetic engineering techniques. The emergence of transgenic crops designed for enhanced zinc uptake and accumulation is examined, providing insights into the potential of genetic modifications to address zinc deficiencies. Additionally, microbial-assisted strategies involving plant-microbe interactions for improved zinc bioavailability are explored. The integration of these diverse biofortification strategies holds promise for mitigating zinc deficiencies at both the agricultural and human levels. By understanding the interplay between soil, plant, and human zinc status, this review aims to provide a comprehensive foundation for future research and the development of sustainable solutions to combat zinc deficiency and improve global nutritional outcomes.</p>