<p>The rising atmospheric CO₂ concentration and temperature under climate change can alter the primary and secondary metabolism of plants. This study aims to investigate the production and active ingredients of <i>Pseudostellaria heterophylla</i> in biochar amended soil under joint treatments of CO₂ enrichment and higher temperature. Series of pot tests were conducted in the control (i.e., 400&#xa0;ppm CO₂, 23.5℃) and rising CO₂ concentration and temperature (i.e., 1000&#xa0;ppm CO₂, 28.5℃) chambers in non-biochar and biochar-treated soils, respectively. Among all the conditions, 3% biochar treatment without rising CO₂ concentration and temperature induced the highest production of the medicinal organ (root tuber) of <i>P. heterophylla</i>. The rising CO₂ and temperature reduced the production of <i>P. heterophylla</i> by up to 87% in the absence of biochar (<i>p</i> &lt; 0.05). Under biochar treatment, it decreased the fresh production by 68% and the dry production by 73%. Moreover, rising CO₂ concentration and temperature as well as biochar treatment had a non-significant negative impact on the concentration of polysaccharides in <i>P. heterophylla</i> root tuber. However, the rising CO₂ concentration and temperature, particularly in the absence of biochar amendment, improved the synthesis of saponins by up to 25% (<i>p</i> &lt; 0.05). This indicates an enhanced quality of <i>P. heterophylla</i> in future climate change. This study reveals that biochar could mitigate the negative effects of future climate change on the production of <i>P. heterophylla</i>. The results of this study could supply the valuable basis for future agriculture practices.</p>