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Purpose <p>Soil salinity negatively modulates plant growth and physio-biochemical attributes. The present study deciphered the <i>Burkholderia</i> sp. AQ12-mediated responses in cotton plants.</p> Methods <p>Seeds of FH-941 (salt sensitive) and FH-326 (tolerant) genotypes were inoculated using AQ12 or water. Half of the both (inoculated and un-inoculated) received no salt while other received salt/NaCl stress. The raised plants were analyzed for various bio-chemical indicators.</p> Results <p>In presence of salt stress, chlorophyll (chl) <i>a</i> content (24.68&#xa0;mg&#xa0;g<sup>−1</sup> FW) in FH-941 was 11.3% and 8.6% less in comparison to its control group and chl <i>a</i> content in FH-326, respectively. FH-941 exhibited a higher oxidant response as indicated by 8.69 and 25.54&#xa0;µmol&#xa0;g<sup>−1</sup> FW malondialdehyde (MDA) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), respectively. A balanced catalase (CAT) and peroxidase (POD) activity across all treatments particularly under salt stress&#xa0;(23.82 and 40.98 U mg<sup>−1</sup> protein, respectively) was recorded in FH-326 indicating its potential to cope NaCl induced oxidative injury. Further, FH-326 exhibited 17.58&#xa0;mg&#xa0;g<sup>−1</sup> DW&#xa0;root K<sup>+</sup> associated with balanced expression of <i>NHX1</i> and <i>HAK5</i>. A higher expression of <i>HKT1</i> but reduced expression of <i>NHX1</i>, <i>SOS1</i> and <i>HAK5</i> indicted poor salt tolerance by FH-941. However, AQ12 inoculation resulted in optimum chl <i>a</i> (28.66&#xa0;mg&#xa0;g<sup>−1</sup> FW), chl <i>b</i> (13.93&#xa0;mg&#xa0;g<sup>−1</sup> FW), MDA (6.89&#xa0;µmol&#xa0;g<sup>−1</sup> FW), H<sub>2</sub>O<sub>2</sub> (20.11&#xa0;µmol&#xa0;g<sup>−1</sup> FW) content in FH-941 resulting in better growth in saline soil.</p> Conclusions <p>The findings highlighted the biochemical and molecular basis of salt tolerance in cotton and signify the importance of AQ12 inoculation for cotton cultivation in saline soils.</p>

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Burkholderia sp. AQ12-mediated Molecular Adjustments Assist Growth of Salt-stressed Cotton (Gossypium hirsutum L.) By Modulating Biochemical Status and Nutrient Acquisition Patterns

  • 期刊论文

  • 发表日期：

    2025年2月3日

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  • Sidra Batool,
  • Sijal Fatima,
  • Uzma Qaisar,
  • Muhammad Shahid,
  • Muhammad Sohail Akram

摘要

Purpose

Soil salinity negatively modulates plant growth and physio-biochemical attributes. The present study deciphered the Burkholderia sp. AQ12-mediated responses in cotton plants.

Methods

Seeds of FH-941 (salt sensitive) and FH-326 (tolerant) genotypes were inoculated using AQ12 or water. Half of the both (inoculated and un-inoculated) received no salt while other received salt/NaCl stress. The raised plants were analyzed for various bio-chemical indicators.

Results

In presence of salt stress, chlorophyll (chl) a content (24.68 mg g−1 FW) in FH-941 was 11.3% and 8.6% less in comparison to its control group and chl a content in FH-326, respectively. FH-941 exhibited a higher oxidant response as indicated by 8.69 and 25.54 µmol g−1 FW malondialdehyde (MDA) and hydrogen peroxide (H2O2), respectively. A balanced catalase (CAT) and peroxidase (POD) activity across all treatments particularly under salt stress (23.82 and 40.98 U mg−1 protein, respectively) was recorded in FH-326 indicating its potential to cope NaCl induced oxidative injury. Further, FH-326 exhibited 17.58 mg g−1 DW root K+ associated with balanced expression of NHX1 and HAK5. A higher expression of HKT1 but reduced expression of NHX1, SOS1 and HAK5 indicted poor salt tolerance by FH-941. However, AQ12 inoculation resulted in optimum chl a (28.66 mg g−1 FW), chl b (13.93 mg g−1 FW), MDA (6.89 µmol g−1 FW), H2O2 (20.11 µmol g−1 FW) content in FH-941 resulting in better growth in saline soil.

Conclusions

The findings highlighted the biochemical and molecular basis of salt tolerance in cotton and signify the importance of AQ12 inoculation for cotton cultivation in saline soils.