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<p>The citrus longhorned beetle (CLB), <i>Anoplophora chinensis</i> Forster (Coleoptera: Cerambycidae), is a highly destructive invasive pest that causes severe economic and ecological damage to more than 100 plant species. Despite extensive eradication actions, quarantine regulations, and chemical control efforts, conventional strategies have proven ineffective in reducing CLB populations, highlighting the urgent need for alternative methods, such as entomopathogenic fungi (EPF), for sustainable management. In this study, the pathogenicity of 21 hypocrealean EPF isolates–primarily <i>Beauveria bassiana</i> (<i>n</i> = 15), <i>Metarhizium anisopliae</i> (4), <i>Cordyceps confragosa</i> (1), and <i>Purpureocillium lilacinum</i> (1)–was evaluated against adult CLB along with a commercial <i>B. bassiana</i> strain (Bb-1) under laboratory conditions. Additionally, eight <i>B. bassiana</i> isolates, including three tested in bioassays, were obtained from CLB adults. Bioassays were conducted using conidial suspensions at a concentration of 1 × 10<sup>8</sup> conidia mL<sup>−1</sup>. Daily observations revealed that cumulative mortality rates ranged from 46.67% to 93.33%, with the highest values recorded for <i>M. anisopliae</i> YK41 (93.33%), YK40 (91.11%), YK35 (91.11%), and <i>B. bassiana</i> YK16 (91.11%). Mycosis rates varied significantly, peaking at a maximum of 95.56% with <i>B. bassiana</i> YK7. Probit analysis indicated LT<sub>50</sub> values of 8.49–14.55 days and LT<sub>90</sub> values of 13.37–21.73 days, with the most rapid effects recorded for <i>M. anisopliae</i> YK40 (LT<sub>50</sub>: 8.49 days and LT<sub>90</sub>: 13.37 days) and <i>B. bassiana</i> YK7 (LT<sub>50</sub>: 9.28 days and LT<sub>90</sub>: 13.68 days). Overall, our findings support the potential of specific EPF as environmentally friendly tools against CLB, although field-based trials are required to validate their efficacy and optimize their use in integrated management strategies.</p>

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Virulence and molecular characterization of native entomopathogenic fungi against Anoplophora chinensis Forster (Coleoptera: Cerambycidae)

  • 期刊论文

  • 发表日期：

    2026年3月30日

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  • Furkan Dogan,
  • Salih Karabörklü,
  • Göksel Özer,
  • Celal Tuncer,
  • I. Oguz Ozdemir

摘要

The citrus longhorned beetle (CLB), Anoplophora chinensis Forster (Coleoptera: Cerambycidae), is a highly destructive invasive pest that causes severe economic and ecological damage to more than 100 plant species. Despite extensive eradication actions, quarantine regulations, and chemical control efforts, conventional strategies have proven ineffective in reducing CLB populations, highlighting the urgent need for alternative methods, such as entomopathogenic fungi (EPF), for sustainable management. In this study, the pathogenicity of 21 hypocrealean EPF isolates–primarily Beauveria bassiana (n = 15), Metarhizium anisopliae (4), Cordyceps confragosa (1), and Purpureocillium lilacinum (1)–was evaluated against adult CLB along with a commercial B. bassiana strain (Bb-1) under laboratory conditions. Additionally, eight B. bassiana isolates, including three tested in bioassays, were obtained from CLB adults. Bioassays were conducted using conidial suspensions at a concentration of 1 × 108 conidia mL−1. Daily observations revealed that cumulative mortality rates ranged from 46.67% to 93.33%, with the highest values recorded for M. anisopliae YK41 (93.33%), YK40 (91.11%), YK35 (91.11%), and B. bassiana YK16 (91.11%). Mycosis rates varied significantly, peaking at a maximum of 95.56% with B. bassiana YK7. Probit analysis indicated LT50 values of 8.49–14.55 days and LT90 values of 13.37–21.73 days, with the most rapid effects recorded for M. anisopliae YK40 (LT50: 8.49 days and LT90: 13.37 days) and B. bassiana YK7 (LT50: 9.28 days and LT90: 13.68 days). Overall, our findings support the potential of specific EPF as environmentally friendly tools against CLB, although field-based trials are required to validate their efficacy and optimize their use in integrated management strategies.