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<p>Leafhoppers are key pests responsible for significant yield losses in important agricultural crops, including okra and cotton in India. These pests cause severe damage to okra, particularly under warmer climatic conditions. A comprehensive study was conducted to assess the genetic diversity and population structure of <i>Amrasca biguttula</i> (Ishida) infesting okra across twenty-six locations spanning three distinct agroecological zones in the state of Chhattisgarh, India using the mitochondrial COI gene. Genetic analysis revealed a Tajima’s D value of 5.5007 (P-value: 8.00087e-17), two parsimony-informative sites, a nucleotide diversity (π) of 0.115, and 81 segregating sites. Population analysis using reticulate trees identified seven low-diversity haplotypes, with CLHH 7 (Chhattisgarh Leafhopper Haplotype 7) being the most dominant across Chhattisgarh. Neighbor-Joining phylogenetic analysis indicated that the overall population is primarily monophyletic except for six populations from central and southern Chhattisgarh, which are paraphyletic. The mean genetic diversity of <i>A. biguttula</i> on okra was very low across the three geographical zones. The very low nucleotide substitution rate in COI sequences suggests a limited mutational risk within <i>A. biguttula</i> populations in Chhattisgarh. This study reports very low genetic diversity among <i>A. biguttula</i> populations on okra, identifying seven haplotypes, with CLHH7 being the most predominant.</p>

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Mitochondrial COI-based genetic diversity of the leafhopper Amrasca biguttula (Ishida) populations in okra in Chhattisgarh state of India

  • 期刊论文

  • 发表日期：

    2025年12月22日

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  • Sneha Sharma,
  • Mallikarjuna Jeer,
  • Kalyan K. Mondal,
  • Kailash Chandra Sharma,
  • Jandrajupalli Sridhar

摘要

Leafhoppers are key pests responsible for significant yield losses in important agricultural crops, including okra and cotton in India. These pests cause severe damage to okra, particularly under warmer climatic conditions. A comprehensive study was conducted to assess the genetic diversity and population structure of Amrasca biguttula (Ishida) infesting okra across twenty-six locations spanning three distinct agroecological zones in the state of Chhattisgarh, India using the mitochondrial COI gene. Genetic analysis revealed a Tajima’s D value of 5.5007 (P-value: 8.00087e-17), two parsimony-informative sites, a nucleotide diversity (π) of 0.115, and 81 segregating sites. Population analysis using reticulate trees identified seven low-diversity haplotypes, with CLHH 7 (Chhattisgarh Leafhopper Haplotype 7) being the most dominant across Chhattisgarh. Neighbor-Joining phylogenetic analysis indicated that the overall population is primarily monophyletic except for six populations from central and southern Chhattisgarh, which are paraphyletic. The mean genetic diversity of A. biguttula on okra was very low across the three geographical zones. The very low nucleotide substitution rate in COI sequences suggests a limited mutational risk within A. biguttula populations in Chhattisgarh. This study reports very low genetic diversity among A. biguttula populations on okra, identifying seven haplotypes, with CLHH7 being the most predominant.