<p>The increase in average temperature due to global climate change induces diverse physiological and biochemical variations in insect pests. These variations influence the cell metabolism, often leading to an increased assemblage of reactive oxygen species (ROS). The brown planthopper (BPH), <i>Nilaparvata lugens</i>, is one of the most destructive insect pests of rice in Asia and Southeast Asia. Temperature plays a critical role in influencing the life history traits of this pest, which are essential for its survival. In these studies, we investigated the influence of temperature variations on nymph survival and development duration under five different temperature combinations. Under these temperature combinations, we also investigated the response of nymphal antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), glutathione reductase (GR) as well as oxidative stress markers such as malondialdehyde (MDA) and hydrogen peroxide (H₂O₂). Our results indicated that the optimal temperature range for nymph survival lies between 27:12 and 33:19&#xa0;°C, where the highest survival rates were observed. In contrast, nymphs exposed to temperature combinations of 23:9, 36:23 and 39:27&#xa0;°C exhibited elevated levels of cumulative stress response index (CSRI), suggesting increased physiological stress under these conditions. This indicates that nymph exhibit greater tolerance to both higher and lower temperature stress through elevated levels of antioxidant enzymes and oxidative stress markers, which likely contributes to enhanced levels of physiological defense under such conditions.</p>