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<p>The two-spotted cricket <i>Gryllus bimaculatus</i> poses growing ecological and agricultural challenges due to its non-diapause behavior and sporadic outbreaks in sensitive environments. This study provides a comprehensive assessment of four pest control agents—Primiphose-methyl, D-tetramethrin, <i>Bacillus thuringiensis</i>, and Chlorfluazuron—through an integrative framework combining toxicity bioassays, morphological evaluation, and molecular docking analysis. Mortality data were statistically analyzed using probit analysis via Ldp-line software to determine LC₅₀ and LC₉₀ values, while regression parameters, chi-square goodness-of-fit, and correlation coefficients were used to validate model reliability. Laboratory results demonstrated that Primiphose-methyl exhibited the highest acute toxicity (LC₅₀ = 2.47 ppm), while Chlorfluazuron caused developmental abnormalities, reflecting its disruption of chitin synthesis pathways. Morphological changes were observed 24&#xa0;h post-treatment and confirmed distinct action patterns across compounds, including exoskeletal deformities and gut distension. In silico docking studies validated these findings, revealing strong binding affinities of Chlorfluazuron to key resistance-related proteins (GST, LCP, and SP), followed by D-tetramethrin and Primiphose-methyl. The integrated use of laboratory assays and computational simulations underscores the value of combining fast-acting neurotoxic insecticides with slower, development-targeting biocontrol agents. This dual approach enhances the sustainability of pest management strategies by mitigating resistance development and minimizing environmental impact. Statistical analyses included probit modeling, chi-square testing, and correlation assessment to confirm dose-response accuracy. The findings offer novel mechanistic insights and practical guidance for the targeted and environmentally responsible control of <i>G. bimaculatus</i> populations.</p>

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Sustainable management of Gryllus bimaculatus: comparative toxicity assessment and molecular docking-based mechanistic validation

  • 期刊论文

  • 发表日期：

    2025年9月11日

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  • Omer M. B. Al-Osimi,
  • Abdullah G. Alghamdi,
  • Habeeb M. Al-Solami,
  • Mohammad. M. Aljameeli,
  • Hayat S. Al-Rashidi,
  • Ahmed H. Abdelwahab,
  • Ahmed S. Hashem,
  • Jazem A. Mahyoub

摘要

The two-spotted cricket Gryllus bimaculatus poses growing ecological and agricultural challenges due to its non-diapause behavior and sporadic outbreaks in sensitive environments. This study provides a comprehensive assessment of four pest control agents—Primiphose-methyl, D-tetramethrin, Bacillus thuringiensis, and Chlorfluazuron—through an integrative framework combining toxicity bioassays, morphological evaluation, and molecular docking analysis. Mortality data were statistically analyzed using probit analysis via Ldp-line software to determine LC₅₀ and LC₉₀ values, while regression parameters, chi-square goodness-of-fit, and correlation coefficients were used to validate model reliability. Laboratory results demonstrated that Primiphose-methyl exhibited the highest acute toxicity (LC₅₀ = 2.47 ppm), while Chlorfluazuron caused developmental abnormalities, reflecting its disruption of chitin synthesis pathways. Morphological changes were observed 24 h post-treatment and confirmed distinct action patterns across compounds, including exoskeletal deformities and gut distension. In silico docking studies validated these findings, revealing strong binding affinities of Chlorfluazuron to key resistance-related proteins (GST, LCP, and SP), followed by D-tetramethrin and Primiphose-methyl. The integrated use of laboratory assays and computational simulations underscores the value of combining fast-acting neurotoxic insecticides with slower, development-targeting biocontrol agents. This dual approach enhances the sustainability of pest management strategies by mitigating resistance development and minimizing environmental impact. Statistical analyses included probit modeling, chi-square testing, and correlation assessment to confirm dose-response accuracy. The findings offer novel mechanistic insights and practical guidance for the targeted and environmentally responsible control of G. bimaculatus populations.