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<p>Development of the internal organs inside the pupal cuticle have the potential to be used as an age marker for blowfly pupae. Internal morphological analysis of different pupal stages by micro-computed tomography (µCT) provides far-reaching internal development deeds, allowing more veracious age and postmortem interval estimation (PMI).The current study was focused on scrutinizing the internal morphological revamp of <i>Chrysomya megacephala</i> (Diptera: calliphoridae) pupae by using synchrotron-based phase-contrast <b>micro-computed tomography</b>. Preeminent age markers were identified and employed to determine if the pupae belong to the early, mid, or pharate stage. The presence of gas bubbles and quantitative changes in organ mass and dorso-longitudinal muscle volume were designated as potential age marker for prepupa, midpupa, and pharate, respectively. For the first time in the forensic entomology world, pupal morphological changes were evaluated through synchrotron based hard X-rays.</p>

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Micro-CT based internal morphological analysis of blowfly (Chrysomya megacephala): a potential tool to age blowfly pupae having forensic implications

  • 期刊论文

  • 发表日期：

    2025年5月31日

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  • Anika Sharma,
  • Ashish Kumar Agrawal,
  • Balwant Singh,
  • Sunil Kumar,
  • Axel Brockmann,
  • Madhu Bala

摘要

Development of the internal organs inside the pupal cuticle have the potential to be used as an age marker for blowfly pupae. Internal morphological analysis of different pupal stages by micro-computed tomography (µCT) provides far-reaching internal development deeds, allowing more veracious age and postmortem interval estimation (PMI).The current study was focused on scrutinizing the internal morphological revamp of Chrysomya megacephala (Diptera: calliphoridae) pupae by using synchrotron-based phase-contrast micro-computed tomography. Preeminent age markers were identified and employed to determine if the pupae belong to the early, mid, or pharate stage. The presence of gas bubbles and quantitative changes in organ mass and dorso-longitudinal muscle volume were designated as potential age marker for prepupa, midpupa, and pharate, respectively. For the first time in the forensic entomology world, pupal morphological changes were evaluated through synchrotron based hard X-rays.