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<p>RNA interference (RNAi) has become a popular tool to analyze gene functions and develop novel strategies to control several insect pests and can be applied to pepper weevil (<i>Anthonomus eugenii</i> Cano), a major pest of pepper crops in the United States, Mexico, and the Caribbean. The larval stages of this pest develop within the fruit, limiting the effective management through conventional and biorational insecticides. The present study investigated the potential of orally delivered double-stranded RNA (dsRNA) targeting pepper weevil heat shock protein 70 (Hsp70) and arginine kinase (ArgK) in controlling this devastating pest. The in vitro synthesized dsRNA was orally administered through an artificial diet comprising a 10% sugar solution. Our result showed that silencing <i>Hsp70</i> and <i>ArgK</i> resulted in mortality of 61.67 and 48.33%, respectively in adult pepper weevils within 10 days. Exposure to dsRNA for 72&#xa0;h significantly suppressed the relative mRNA expression of <i>Hsp70</i> and <i>ArgK</i> by 4.15 and 5.06-fold, respectively. Our results showed an effective dietary RNAi response in pepper weevil and <i>Hsp70</i> and <i>ArgK</i> are potential RNAi target genes for controlling this destructive pest. Upon validation in the field, these genes could provide sustainable solutions to control pepper weevil.</p>

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Targeted RNAi of Hsp70 and ArgK genes via oral DsRNA delivery in pepper weevil (Anthonomus eugenii)

  • 期刊论文

  • 发表日期：

    2025年2月26日

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  • Sumit Jangra,
  • Naga Mani Kanchupati,
  • Dakshina R. Seal,
  • Romina Gazis

摘要

RNA interference (RNAi) has become a popular tool to analyze gene functions and develop novel strategies to control several insect pests and can be applied to pepper weevil (Anthonomus eugenii Cano), a major pest of pepper crops in the United States, Mexico, and the Caribbean. The larval stages of this pest develop within the fruit, limiting the effective management through conventional and biorational insecticides. The present study investigated the potential of orally delivered double-stranded RNA (dsRNA) targeting pepper weevil heat shock protein 70 (Hsp70) and arginine kinase (ArgK) in controlling this devastating pest. The in vitro synthesized dsRNA was orally administered through an artificial diet comprising a 10% sugar solution. Our result showed that silencing Hsp70 and ArgK resulted in mortality of 61.67 and 48.33%, respectively in adult pepper weevils within 10 days. Exposure to dsRNA for 72 h significantly suppressed the relative mRNA expression of Hsp70 and ArgK by 4.15 and 5.06-fold, respectively. Our results showed an effective dietary RNAi response in pepper weevil and Hsp70 and ArgK are potential RNAi target genes for controlling this destructive pest. Upon validation in the field, these genes could provide sustainable solutions to control pepper weevil.