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<p><i>Amrasca biguttula</i>, commonly known as the cotton jassid, is a major sap-sucking pest of cotton, which causes chlorosis, leaf curling, and hopper burn leading to the stunted plant growth and reduced yield. However, over-reliance on insecticide to manage this pest contributes to resistance development and raises environmental concerns. Insecticidal and biochemical assays were carried out from nine districts of Pakistan to monitor resistance of commonly used insecticides against <i>A. biguttula</i>. The field resistance levels recorded were 10.31-41.23-fold to profenofos, 10.07-83.57-fold to chlorpyrifos, 8.70-30.02-fold to bifenthrin, 4.56-26.24-fold to deltamethrin, 56.28-218.16-fold to imidacloprid, 5.70-23.30-fold to fipronil, 2.99-18.26-fold to clothianidin, 4.66-25.43-fold to chlorfenapyr, and 5.33-44.51-fold to carbosulfan as compared to the susceptible population. Elevated levels of acid and alkaline phosphatases were recorded as 43.18 and 45.25 µmol/min/mg protein in clothianidin and deltamethrin treated population, respectively. In the case of esterases the highest activity i.e., 63.79 µmol/min/mg protein was noted in carbosulfan exposed population. Maximum level of glutathione S-transferase was recorded as 21.99 µmol/min/mg protein in fipronil treated population. Clothianidin treated population showed increased level of acetyl cholinesterase i.e., 46.22 µmol/min/mg. Increased activities of detoxification enzymes can possibly be the cause of resistance development against insecticides. Integrated pest management techniques including cultural practices, avoiding excessive and repeated use of insecticides can aid for the management of <i>A. biguttula.</i></p>

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Monitoring and biochemical characterization of resistance against commonly used insecticides in cotton jassid, Amrasca biguttula (Ishida) from Punjab, Pakistan

  • 期刊论文

  • 发表日期：

    2025年1月29日

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  • Sikandar Hussain,
  • Shoaib Freed,
  • Afifa Naeem,
  • Muhammad Sheraz Shah,
  • Muhammad Zeeshan Nazar,
  • Muhammad Waqas Sumra

摘要

Amrasca biguttula, commonly known as the cotton jassid, is a major sap-sucking pest of cotton, which causes chlorosis, leaf curling, and hopper burn leading to the stunted plant growth and reduced yield. However, over-reliance on insecticide to manage this pest contributes to resistance development and raises environmental concerns. Insecticidal and biochemical assays were carried out from nine districts of Pakistan to monitor resistance of commonly used insecticides against A. biguttula. The field resistance levels recorded were 10.31-41.23-fold to profenofos, 10.07-83.57-fold to chlorpyrifos, 8.70-30.02-fold to bifenthrin, 4.56-26.24-fold to deltamethrin, 56.28-218.16-fold to imidacloprid, 5.70-23.30-fold to fipronil, 2.99-18.26-fold to clothianidin, 4.66-25.43-fold to chlorfenapyr, and 5.33-44.51-fold to carbosulfan as compared to the susceptible population. Elevated levels of acid and alkaline phosphatases were recorded as 43.18 and 45.25 µmol/min/mg protein in clothianidin and deltamethrin treated population, respectively. In the case of esterases the highest activity i.e., 63.79 µmol/min/mg protein was noted in carbosulfan exposed population. Maximum level of glutathione S-transferase was recorded as 21.99 µmol/min/mg protein in fipronil treated population. Clothianidin treated population showed increased level of acetyl cholinesterase i.e., 46.22 µmol/min/mg. Increased activities of detoxification enzymes can possibly be the cause of resistance development against insecticides. Integrated pest management techniques including cultural practices, avoiding excessive and repeated use of insecticides can aid for the management of A. biguttula.