Scholarlink

<p>The rise in antimicrobial resistance, free radical induced oxidative stress and exposure to toxic agents are major health concerns demand the need for safter and less harmful medications for efficient treatments. In this context, this research delves into the benign production of silver nanoparticles (AgNPs) utilizing leaf extract of <i>Ipomoea sepiaria</i> (IS) and evaluates their bioactive potential. UV–Vis analysis of the IS AgNPs revealed a distinct absorption peak at 410&#xa0;nm. XRD results indicated the crystalline structure of the synthesized IS AgNPs, measuring 9.32&#xa0;nm in size. HR-TEM analysis showed that IS AgNPs were predominantly spherical, with an average diameter of 36 ± 9.44&#xa0;nm. FTIR analysis revealed that, aromatic amines, alkanes, alkenes, ketones, sulfone and alcohol groups were the key agents involved in the formation and stabilization of AgNPs. The antibacterial activity of the IS AgNPs against test bacterial pathogens demonstrated varying degrees of the inhibitory zones. Notably, it rendered a profound halo measuring 18.3&#xa0;mm with a Minimum Inhibitory Concentration (MIC) of 32&#xa0;µg/ml and a Minimum Bactericidal Concentration (MBC) of 64&#xa0;µg/ml against <i>E. coli.</i> The ability of IS AgNPs to scavenge DPPH radicals showed a dose-dependent increase ranged between 8.1% (20&#xa0;µg) and 81% (100&#xa0;µg). Cytotoxicity using THP1 cell line revealed moderate cytotoxicity with an IC50 value of 47.44&#xa0;µg/ ml. The acute toxicity assessment of IS AgNPs against zebrafish <i>Danio rerio</i> model showed no signs of mortality or toxic behaviour after 96-h exposure at 50&#xa0;µg/l. Furthermore, the <i>in vivo</i> wound healing study revealed that 50&#xa0;µg/l of IS AgNPs efficiently accelerated the wound closure than the untreated controls, with histopathological evaluations confirming enhanced tissue regeneration.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Plant-mediated fabrication of silver nanoparticles from Ipomoea sepiaria leaves: evaluation of biomedical potentials

  • 期刊论文

  • 发表日期：

    2026年3月27日

查看全文
  • Bharathi Selvaraj,
  • Deepa Chokkalingam,
  • Devagi Poongazhalselvan,
  • Anbupriya Marimuthu,
  • Kavitha Kuppusamy,
  • Rajalakshmi Arumugam,
  • Ramesh Balasubramanian,
  • Saravanan Durai,
  • Suresh Gopal

摘要

The rise in antimicrobial resistance, free radical induced oxidative stress and exposure to toxic agents are major health concerns demand the need for safter and less harmful medications for efficient treatments. In this context, this research delves into the benign production of silver nanoparticles (AgNPs) utilizing leaf extract of Ipomoea sepiaria (IS) and evaluates their bioactive potential. UV–Vis analysis of the IS AgNPs revealed a distinct absorption peak at 410 nm. XRD results indicated the crystalline structure of the synthesized IS AgNPs, measuring 9.32 nm in size. HR-TEM analysis showed that IS AgNPs were predominantly spherical, with an average diameter of 36 ± 9.44 nm. FTIR analysis revealed that, aromatic amines, alkanes, alkenes, ketones, sulfone and alcohol groups were the key agents involved in the formation and stabilization of AgNPs. The antibacterial activity of the IS AgNPs against test bacterial pathogens demonstrated varying degrees of the inhibitory zones. Notably, it rendered a profound halo measuring 18.3 mm with a Minimum Inhibitory Concentration (MIC) of 32 µg/ml and a Minimum Bactericidal Concentration (MBC) of 64 µg/ml against E. coli. The ability of IS AgNPs to scavenge DPPH radicals showed a dose-dependent increase ranged between 8.1% (20 µg) and 81% (100 µg). Cytotoxicity using THP1 cell line revealed moderate cytotoxicity with an IC50 value of 47.44 µg/ ml. The acute toxicity assessment of IS AgNPs against zebrafish Danio rerio model showed no signs of mortality or toxic behaviour after 96-h exposure at 50 µg/l. Furthermore, the in vivo wound healing study revealed that 50 µg/l of IS AgNPs efficiently accelerated the wound closure than the untreated controls, with histopathological evaluations confirming enhanced tissue regeneration.