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<p>The objective of this study is to ascertain the biological potencies of the various fractions obtained from the aerial part of <i>Gymnocarpos decander</i> Forsk (El djefna). The estimation of polyphenols and flavonoids was conducted. The antimicrobial effect was carried out in accordance with the CLSI recommendations, employing a range of reference microorganisms. Meanwhile, the antioxidant potency was determined using means of the DPPH, ABTS and FRAP techniques. The hemolytic activity was determined, and the interaction between bioactive substances with SAP2 and SAP3 was investigated using molecular docking. The results indicate that the ethyl acetate fraction contains high levels of polyphenols and flavonoids (17.587 µEAG/mg MS and 25.456 µEC/mg MS respectively). The antimicrobial effects indicate that the hexanic fraction has the potential activity against <i>L. monocytogenes</i> ATCC 15,313 and <i>Bacillus subtilis</i> ATCC 6633 with a concentration of 2.5&#xa0;mg/mL. However, it has been demonstrated that the ethyl acetate fraction is particularly effective against <i>Candida albicans</i> IP444 with a MIC of 5&#xa0;mg/mL. The antioxidant activity shows that ethyl acetate and hexanic fractions have better effects in which the ABTS, FRAP and DPPH assays were values ranged between 3.22&#xa0;µg/mL and 7.82&#xa0;µg/mL. The hemolytic test revealed that none of the fractions are toxic, and the molecular docking suggested that El djefna could be useful in developing new drugs.</p>

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In vitro biological effects and in silico docking properties of different fractions obtained from Gymnocarpos decander Forsk aerial part collected in the wilaya of Naama

  • 期刊论文

  • 发表日期：

    2025年12月25日

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  • Mohammed Aissaoui,
  • Chachoua Sarra,
  • Bendjerad Manal,
  • Nouari Wafa,
  • Aissaoui Nadia

摘要

The objective of this study is to ascertain the biological potencies of the various fractions obtained from the aerial part of Gymnocarpos decander Forsk (El djefna). The estimation of polyphenols and flavonoids was conducted. The antimicrobial effect was carried out in accordance with the CLSI recommendations, employing a range of reference microorganisms. Meanwhile, the antioxidant potency was determined using means of the DPPH, ABTS and FRAP techniques. The hemolytic activity was determined, and the interaction between bioactive substances with SAP2 and SAP3 was investigated using molecular docking. The results indicate that the ethyl acetate fraction contains high levels of polyphenols and flavonoids (17.587 µEAG/mg MS and 25.456 µEC/mg MS respectively). The antimicrobial effects indicate that the hexanic fraction has the potential activity against L. monocytogenes ATCC 15,313 and Bacillus subtilis ATCC 6633 with a concentration of 2.5 mg/mL. However, it has been demonstrated that the ethyl acetate fraction is particularly effective against Candida albicans IP444 with a MIC of 5 mg/mL. The antioxidant activity shows that ethyl acetate and hexanic fractions have better effects in which the ABTS, FRAP and DPPH assays were values ranged between 3.22 µg/mL and 7.82 µg/mL. The hemolytic test revealed that none of the fractions are toxic, and the molecular docking suggested that El djefna could be useful in developing new drugs.