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<p>There has been a great deal of research in the field of artificial cultivation of black truffles including <i>Tuber huidongense, Tuber melanosporum</i> and <i>Tuber indicum</i>. Here, molecular real time identification of culture at low cost is very important to ensure quality of truffle products. Identification of truffle species based on genus or species-specific primers developed so far has limitations of being laborious and costly, as sequencing or PCR are required. In this study, we constructed in silico virtual PCR environment being able to confirm amplification potential of species-specific markers and conducted molecular discrimination of 3 truffle species by multiplex PCR. Evaluation of amplification potential in the virtual PCR environment and multiplex PCR assay using DNA barcode genes (<i>ITS</i>, <i>β-tubulin</i>, and <i>28S rRNA</i>) would contribute to molecular species identification of truffle species in the future.</p>

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Evaluation of amplification potential of species identification markers in a virtual PCR environment and efficient molecular identification of three black truffle species by multiplex PCR assay

  • 期刊论文

  • 发表日期：

    2025年11月10日

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  • Ju-Il Song,
  • Mi-Ryong Choe,
  • Un-Hyang Ho,
  • Sok-Jun Ri,
  • Mi-Hyang Kim,
  • Tae-Ryong Ri,
  • Chung-Il Yo,
  • Yong-Nam Kim

摘要

There has been a great deal of research in the field of artificial cultivation of black truffles including Tuber huidongense, Tuber melanosporum and Tuber indicum. Here, molecular real time identification of culture at low cost is very important to ensure quality of truffle products. Identification of truffle species based on genus or species-specific primers developed so far has limitations of being laborious and costly, as sequencing or PCR are required. In this study, we constructed in silico virtual PCR environment being able to confirm amplification potential of species-specific markers and conducted molecular discrimination of 3 truffle species by multiplex PCR. Evaluation of amplification potential in the virtual PCR environment and multiplex PCR assay using DNA barcode genes (ITS, β-tubulin, and 28S rRNA) would contribute to molecular species identification of truffle species in the future.