Scholarlink

<p>Ammonium nitrate is an essential salt of MS medium but is banned in the market due to its use in illegal and terrorist activities. In the absence of ammonium nitrate, commercially available plant tissue culture medium is the only option for conducting the plant tissue culture based experiments. However, the availability of the commercial medium is not a proper solution to optimize the micropropagation protocol for all types of plants. Therefore, replacement of the ammonium nitrate with a suitable, low-cost chemical is the need of the hour. In this background, we prepared ammonium nitrate less Experimental MS medium using 1.101&#xa0;g/l ammonium chloride and 1.75&#xa0;g/l sodium nitrate and tested for the in vitro propagation of banana (Grand Naine cultivar). No significant differences were found between the MS medium and Experimental MS (EMS) medium on survival rate and response percentage during the culture initiation phase of banana. In vitro rooting of banana was found under the treatment of EMS medium. In addition to banana, this medium also induced in vitro shoot of <i>Aloe vera</i>. Therefore, these salt combinations may be used as a potent substitute for ammonium nitrate in MS medium.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Assessment of low-cost alternative of ammonium nitrate of MS medium for in vitro propagation of plant

  • 期刊论文

  • 发表日期：

    2025年12月15日

查看全文
  • Syandan Sinha Ray,
  • Manidipa Mondal,
  • Sohan Bhattacharjee,
  • Debasis Bhattacharyya,
  • Gautam Chatterjee,
  • Md. Nasim Ali

摘要

Ammonium nitrate is an essential salt of MS medium but is banned in the market due to its use in illegal and terrorist activities. In the absence of ammonium nitrate, commercially available plant tissue culture medium is the only option for conducting the plant tissue culture based experiments. However, the availability of the commercial medium is not a proper solution to optimize the micropropagation protocol for all types of plants. Therefore, replacement of the ammonium nitrate with a suitable, low-cost chemical is the need of the hour. In this background, we prepared ammonium nitrate less Experimental MS medium using 1.101 g/l ammonium chloride and 1.75 g/l sodium nitrate and tested for the in vitro propagation of banana (Grand Naine cultivar). No significant differences were found between the MS medium and Experimental MS (EMS) medium on survival rate and response percentage during the culture initiation phase of banana. In vitro rooting of banana was found under the treatment of EMS medium. In addition to banana, this medium also induced in vitro shoot of Aloe vera. Therefore, these salt combinations may be used as a potent substitute for ammonium nitrate in MS medium.