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Key message <p><b>An optimized protocol of regeneration was developed for a head cabbage using leaf explants on MS supplemented medium with different concentration and combination of phytohormones. </b></p> Abstract <p>Cabbage, an agriculturally significant vegetable crop renowned for its substantial nutritional and cost-effective value, is experiencing a surge in commercial demand. The global demand creates a necessity for the production of superior planting material. In this study, we established an efficient protocol for regeneration of head cabbage from the leaf explants. We assessed the organogenic potential of the leaf segment on MS basal medium having different plant growth hormones, like Thidiazuron (TDZ) and 6-Benzylaminopurine (BA), either alone or combined with Naphthalene Acetic Acid (NAA), Indole Butyric Acid (IBA), Indole-3-Acetic Acid (IAA), and adenine. BA exhibited a&#xa0;good morphogenic response, with approximately 83.47% of leaf explants producing shoots on MS basal medium having 5&#xa0;mg/l BA and 1.0&#xa0;mg/l NAA. Furthermore, addition of 0.2&#xa0;mg/l NAA and 0.5&#xa0;mg/l TDZ to MS medium enhanced shoot regeneration frequency to 84.45%. However, after 4&#xa0;weeks of light incubation, aberrant, vitrified, and stunted shoots were produced. Subsequent proliferation and elongation of micro-shoots were successfully achieved on MS multiplication medium containing 1&#xa0;mg/l BA, 1&#xa0;mg/l NAA, and 0.25&#xa0;mg/l kinetin. In vitro roots were successfully induced on half-strength MS basal medium + 0.1&#xa0;mg/l IBA, resulting in 83.45% rooting efficiency. Well-rooted plantlets were effectively acclimatized in either pots or trays filled with sterilized cocopeat: perlite: vermiculite (3:1:1) before being transplanted in the soil. The optimized protocol developed in this study can be effectively applicable for efficiently scaling up cabbage propagation and is also essential for enhancing traits via genetic engineering.</p>

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Effect of cytokinins and auxins on plant regeneration from leaf explants in head cabbage (Brassica oleracea var. capitata L.), a highly recommended “nutrient rich” vegetable crop

  • 期刊论文

  • 发表日期：

    2025年7月30日

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  • Palvi Thakur,
  • Neha Kumari,
  • Sanjay Chadha

摘要

Key message

An optimized protocol of regeneration was developed for a head cabbage using leaf explants on MS supplemented medium with different concentration and combination of phytohormones.

Abstract

Cabbage, an agriculturally significant vegetable crop renowned for its substantial nutritional and cost-effective value, is experiencing a surge in commercial demand. The global demand creates a necessity for the production of superior planting material. In this study, we established an efficient protocol for regeneration of head cabbage from the leaf explants. We assessed the organogenic potential of the leaf segment on MS basal medium having different plant growth hormones, like Thidiazuron (TDZ) and 6-Benzylaminopurine (BA), either alone or combined with Naphthalene Acetic Acid (NAA), Indole Butyric Acid (IBA), Indole-3-Acetic Acid (IAA), and adenine. BA exhibited a good morphogenic response, with approximately 83.47% of leaf explants producing shoots on MS basal medium having 5 mg/l BA and 1.0 mg/l NAA. Furthermore, addition of 0.2 mg/l NAA and 0.5 mg/l TDZ to MS medium enhanced shoot regeneration frequency to 84.45%. However, after 4 weeks of light incubation, aberrant, vitrified, and stunted shoots were produced. Subsequent proliferation and elongation of micro-shoots were successfully achieved on MS multiplication medium containing 1 mg/l BA, 1 mg/l NAA, and 0.25 mg/l kinetin. In vitro roots were successfully induced on half-strength MS basal medium + 0.1 mg/l IBA, resulting in 83.45% rooting efficiency. Well-rooted plantlets were effectively acclimatized in either pots or trays filled with sterilized cocopeat: perlite: vermiculite (3:1:1) before being transplanted in the soil. The optimized protocol developed in this study can be effectively applicable for efficiently scaling up cabbage propagation and is also essential for enhancing traits via genetic engineering.