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<p>The exploration of medicinal plants for therapeutic application has been a long standing practice owing to their rich repository of bioactive compounds. This study aimed to analyze the chemical constituents along with anti-oxidant and anti-microbial potential evaluation of 70% hydro-methanolic leaf extract of <i>Aristolochia assamica</i>, an endemic medicinal plant of the East Himalayas (N.E India). Collection of <i>Aristolochia assamica</i> leaves was done from the fringe villages of Behali Reserve Forest present in Biswanath district of Assam. Following authentication, the 70% hydro-methanolic leaf extract (Net Yield = 103&#xa0;mg/g dry weight) was subjected to anti-oxidant potential evaluation (DPPH assay, Total Anti-oxidant assay, NO assay and FRAP assay), anti-microbial assay, FT-IR profiling and LC-MS analysis. The results of DPPH radical scavenging assay and Total Anti-oxidant assay revealed the possession of moderately high DPPH radical scavenging property (IC<sub>50</sub> = 58.63 ± 2.52&#xa0;µg/mL; Standard (Ascorbic Acid) IC<sub>50</sub> = 73.44 ± 0.22&#xa0;µg/mL) and high total anti-oxidant potential with TEAC = 1.159 ± 0.014. Even the NO scavenging assay and FRAP assay demonstrate the moderate anti-oxidant potential of the extract (IC<sub>50</sub> = 109.501 ± 1.033&#xa0;µg/mL in case of NO assay and FRAP value of 9.98 ± 1.079&#xa0;µg TE per mL of extract in case of FRAP assay). The anti-microbial assay revealed the possession of substantial anti-microbial activity against <i>Pseudomonas aeruginosa</i>, <i>Kleibsella pneumoniae</i>, <i>Escherichia coli</i>, <i>Staphylococcus aureus</i> and <i>Enterococcus faecalis</i> with ZOI = 17.67&#xa0;mm, 15.33&#xa0;mm, 19.33&#xa0;mm, 20.33&#xa0;mm and 23.33&#xa0;mm respectively with MIC values within the range of 0.0125-0.05&#xa0;mg/mL. The FT-IR profiling revealed the presence of functional groups like -CH<sub>2</sub>, =C-H, C-N, C-O, C = C, C = N, C = O, C-H, O-H and N-H, the outcome of which is substantiated by LC-MS analysis as it predicts the presence of a total of 16 phyto-constituents like 1-isothiocyanato-4-(methylsulfinyl)-butane, sennoside, papaverine, solasodine, kaempferol-3-O-rutinoside, codeinone, apigenin 7-O-rutinoside which possess those FT-IR predicted functional groups. Henceforth the hydro-methanolic leaf extract possesses high anti-oxidant and anti-microbial activity in turn providing substantial evidence for being a potentially rich source of anti-oxidants and anti-microbials.</p>

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Phyto-chemical profiling, anti-oxidant and anti-microbial potential evaluation of the hydro-methanolic leaf extract of Aristolochia assamica, a new endemic species reported from N.E India

  • 期刊论文

  • 发表日期：

    2025年7月17日

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  • Aashis Dutta,
  • Ranjit Kakati,
  • Nayanabhiram Deka,
  • Aziz Hussain,
  • Raju Ojah,
  • Dipankar Borah,
  • Manas Das

摘要

The exploration of medicinal plants for therapeutic application has been a long standing practice owing to their rich repository of bioactive compounds. This study aimed to analyze the chemical constituents along with anti-oxidant and anti-microbial potential evaluation of 70% hydro-methanolic leaf extract of Aristolochia assamica, an endemic medicinal plant of the East Himalayas (N.E India). Collection of Aristolochia assamica leaves was done from the fringe villages of Behali Reserve Forest present in Biswanath district of Assam. Following authentication, the 70% hydro-methanolic leaf extract (Net Yield = 103 mg/g dry weight) was subjected to anti-oxidant potential evaluation (DPPH assay, Total Anti-oxidant assay, NO assay and FRAP assay), anti-microbial assay, FT-IR profiling and LC-MS analysis. The results of DPPH radical scavenging assay and Total Anti-oxidant assay revealed the possession of moderately high DPPH radical scavenging property (IC50 = 58.63 ± 2.52 µg/mL; Standard (Ascorbic Acid) IC50 = 73.44 ± 0.22 µg/mL) and high total anti-oxidant potential with TEAC = 1.159 ± 0.014. Even the NO scavenging assay and FRAP assay demonstrate the moderate anti-oxidant potential of the extract (IC50 = 109.501 ± 1.033 µg/mL in case of NO assay and FRAP value of 9.98 ± 1.079 µg TE per mL of extract in case of FRAP assay). The anti-microbial assay revealed the possession of substantial anti-microbial activity against Pseudomonas aeruginosa, Kleibsella pneumoniae, Escherichia coli, Staphylococcus aureus and Enterococcus faecalis with ZOI = 17.67 mm, 15.33 mm, 19.33 mm, 20.33 mm and 23.33 mm respectively with MIC values within the range of 0.0125-0.05 mg/mL. The FT-IR profiling revealed the presence of functional groups like -CH2, =C-H, C-N, C-O, C = C, C = N, C = O, C-H, O-H and N-H, the outcome of which is substantiated by LC-MS analysis as it predicts the presence of a total of 16 phyto-constituents like 1-isothiocyanato-4-(methylsulfinyl)-butane, sennoside, papaverine, solasodine, kaempferol-3-O-rutinoside, codeinone, apigenin 7-O-rutinoside which possess those FT-IR predicted functional groups. Henceforth the hydro-methanolic leaf extract possesses high anti-oxidant and anti-microbial activity in turn providing substantial evidence for being a potentially rich source of anti-oxidants and anti-microbials.