<p>Identification of RAPD (Random Amplified Polymorphic DNA) marker for bud fly resistant gene. In the present study, 25 RAPD primers were tested to distinguish the parental lines, F₁ and F₂ lines. However, only 15 RAPD gave scorable and reproducible band. All these markers were found to be reproducible in three repeated RAPD. Data from these scorable primers were converted in to 0–1 data, based on the presence or absence of bands, these 0–1 data were used to calculate, jaccard’s similarity coefficient, upon which UPGMA cluster analysis were performed. The number of bands generated per primer ranged from 3 to 8 with a mean of 5.67 bands per primer. Out of 81 bands generated, 53 (65.43%) were polymorphic and 20 (24.69%) were monomorphic. The similarity coefficient of pair-wise comparisons among genotypes ranged from 0.48 to 1.00. The dendogram, generated from similarity matrix, was based on the principle of UPGMA. However, the dendogram showed only 30 genotypes, it may have happened the tie of some similar values. Based on the Jaccard similarity coefficient, four groups were obtained. The genetic similarity value was highest between Shekhar ×Neela and Neelam×Neela (1.00), whereas Neelam×IC15888 ×JRF, Neelam ×IC15888×Shekhar and Neela ×EC1424×JRF-5 showed the least similarity (0.294). The highest variability was in Neelam, while Neela was the least variable. The similarity coefficient in group I ranged from 0.647 to 0.970. By contrast, the similarity coefficient of group II ranged from 0.588 to 0.970. Group III showed a similarity coefficient range of 0.705–0.970. Group IV showed a similarity coefficient range of 0.911–0.970.</p>