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<p>In vitro laticifer production during callus development was investigated in <i>Hevea brasiliensis</i> Muell. Arg. (RRIM600 cultivar). A seed-derived callus was grown for a month on a Murashige and Skoog medium supplemented with 2&#xa0;mg/L of 2,4-dichlorophenoxyacetic acid, 2&#xa0;mg/L of benzyladenine, and 5% sucrose before being transferred to the same medium with 3% sucrose for proliferation. For three months, the rising fresh weight of these calli was measured weekly in order to investigate their development. The callus generated from seeds followed a sigmoidal pattern, with stationary, exponential, and lag phases. In vitro laticifers were discovered after the second week of cultivation and reached their peak density in 8 weeks. After 12 weeks of culture, all laticifer types including those that had secreted latex, starch granule laticifers, and main laticifers were revealed. The findings revealed that the culture time interval influenced the in vitro proliferation of numerous laticifer types in the seed-derived callus of <i>H. brasiliensis</i>. During the two to eight weeks of cultivation, the density and breadth of laticifer cells in seed-derived callus rose slightly. A correlation between the culture period and the number of in vitro laticifers in seed-derived calli were also found.</p>

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Laticifer development of seed-derived callus of Hevea brasiliensis Muell. Arg

  • 期刊论文

  • 发表日期：

    2025年7月1日

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  • Yurachat Meksuwan,
  • Peerapat Roongsattham,
  • Charatsri Nualsri,
  • Upatham Meesawat

摘要

In vitro laticifer production during callus development was investigated in Hevea brasiliensis Muell. Arg. (RRIM600 cultivar). A seed-derived callus was grown for a month on a Murashige and Skoog medium supplemented with 2 mg/L of 2,4-dichlorophenoxyacetic acid, 2 mg/L of benzyladenine, and 5% sucrose before being transferred to the same medium with 3% sucrose for proliferation. For three months, the rising fresh weight of these calli was measured weekly in order to investigate their development. The callus generated from seeds followed a sigmoidal pattern, with stationary, exponential, and lag phases. In vitro laticifers were discovered after the second week of cultivation and reached their peak density in 8 weeks. After 12 weeks of culture, all laticifer types including those that had secreted latex, starch granule laticifers, and main laticifers were revealed. The findings revealed that the culture time interval influenced the in vitro proliferation of numerous laticifer types in the seed-derived callus of H. brasiliensis. During the two to eight weeks of cultivation, the density and breadth of laticifer cells in seed-derived callus rose slightly. A correlation between the culture period and the number of in vitro laticifers in seed-derived calli were also found.