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<p>Developing a fully functional root system is one measure to promote the survival of date palm plantlets upon transfer to an <i>ex vitro</i> environment. This study aimed to evaluate the effect of different IAA treatments, either alone or in combination with phloroglucinol (PG) and/or melatonin (MEL), on the rooting and acclimatization of <i>P. dactylifera</i>. Rooting percentage, root number and root length were significantly increased at p ≤ 0.05 with 20&#xa0;mg/L PG + 0.5&#xa0;mM MEL + 0.5&#xa0;mg/L IAA. Our findings revealed that the maximum carbohydrates in shoots, endogenous IAA and chlorophyll content were recorded in a medium supplemented with 20&#xa0;mg/L PG + 0.5&#xa0;mM MEL + 0.5&#xa0;mg/L IAA. Data indicated that the highest percentage of plantlet survival (94.11%) during the first 14 weeks of transferring was in plantlets grown in a mixture of peat moss and perlite (3:1). The anatomical study revealed a mechanism for differentiating adventitious roots from shoot bases. Meristemoid cells were distinct, consisting of dense cells with enlarged nuclei. Distinct root primordia were conical in shape and had several cell layers. Root emergence required 45 days after the above treatments. The use of RAPD and ISSR markers showed a consistent banding pattern, confirming the genetic stability of tissue culture-derived plants compared to parent plants. In conclusion, the above treatments positively affected both rooting and acclimatization in <i>P. dactylifera</i>. They can be exploited for this important plant's conservation and widespread propagation.</p>

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Micropropagation of Phoenix dactylifera L using phloroglucinol, melatonin and indole-3-acetic acid

  • 期刊论文

  • 发表日期：

    2025年6月20日

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  • Ahmed Madi Waheed Al–Mayahi

摘要

Developing a fully functional root system is one measure to promote the survival of date palm plantlets upon transfer to an ex vitro environment. This study aimed to evaluate the effect of different IAA treatments, either alone or in combination with phloroglucinol (PG) and/or melatonin (MEL), on the rooting and acclimatization of P. dactylifera. Rooting percentage, root number and root length were significantly increased at p ≤ 0.05 with 20 mg/L PG + 0.5 mM MEL + 0.5 mg/L IAA. Our findings revealed that the maximum carbohydrates in shoots, endogenous IAA and chlorophyll content were recorded in a medium supplemented with 20 mg/L PG + 0.5 mM MEL + 0.5 mg/L IAA. Data indicated that the highest percentage of plantlet survival (94.11%) during the first 14 weeks of transferring was in plantlets grown in a mixture of peat moss and perlite (3:1). The anatomical study revealed a mechanism for differentiating adventitious roots from shoot bases. Meristemoid cells were distinct, consisting of dense cells with enlarged nuclei. Distinct root primordia were conical in shape and had several cell layers. Root emergence required 45 days after the above treatments. The use of RAPD and ISSR markers showed a consistent banding pattern, confirming the genetic stability of tissue culture-derived plants compared to parent plants. In conclusion, the above treatments positively affected both rooting and acclimatization in P. dactylifera. They can be exploited for this important plant's conservation and widespread propagation.