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<p><i>Coccinia grandis,</i> a perennial climbing plant used in traditional medicine, produces 2-methoxy-4-vinylphenol, a secondary metabolite crucial for inhibiting pancreatic cancer cell growth. This study aimed to enhance 2M4VP biosynthesis in <i>C. grandis</i> cell suspension cultures (CSCs) by evaluating the individual effects of the elicitors chitosan, methyl jasmonate, salicylic acid, and zinc oxide nanoparticles (ZnO NPs). The ideal concentration of 2.0&#xa0;mg/L 2,4-D in Murashige and Skoog&#xa0;(MS) medium induced 100% white friable callus (FC) formation from <i>C. grandis</i> nodal segments. CSCs were established using FC in liquid MS with 2,4-D at 2.0&#xa0;mg/L. Among all treatments, 15&#xa0;mg/L ZnO NPs yielded the highest biomass, with 14.176&#xa0;g fresh weight (FW) and 2.476&#xa0;g dry weight (DW). The highest 2M4VP production was also observed at this concentration of ZnO NPs, reaching 7.84&#xa0;mg/g DW, representing an 8.17-fold increase. ZnO NPs also significantly enhanced antioxidant activity, with increases of 1.36-fold in DPPH, 1.92-fold in HRSA, and 1.03-fold in ABTS<sup>+</sup> compared to the controls. Applying ZnO NPs in plant tissue culture presents a promising strategy for enhancing 2M4VP production and antioxidant activity in <i>C. grandis</i>, underscoring their potential for pharmaceutical applications.</p>

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ZnO nanoparticles and elicitors in 2M4VP enrichment and antioxidant capacity in cell suspension cultures of Coccinia grandis (L.) Voigt

  • 期刊论文

  • 发表日期：

    2025年5月25日

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  • Ananthakumar Archana,
  • Shanthi Pandurengan Parthasarathy,
  • Senguttuvan Vignesh,
  • Chinnasamy Appunu,
  • Subramaniyam Alagumanian,
  • Markandan Manickavasagam

摘要

Coccinia grandis, a perennial climbing plant used in traditional medicine, produces 2-methoxy-4-vinylphenol, a secondary metabolite crucial for inhibiting pancreatic cancer cell growth. This study aimed to enhance 2M4VP biosynthesis in C. grandis cell suspension cultures (CSCs) by evaluating the individual effects of the elicitors chitosan, methyl jasmonate, salicylic acid, and zinc oxide nanoparticles (ZnO NPs). The ideal concentration of 2.0 mg/L 2,4-D in Murashige and Skoog (MS) medium induced 100% white friable callus (FC) formation from C. grandis nodal segments. CSCs were established using FC in liquid MS with 2,4-D at 2.0 mg/L. Among all treatments, 15 mg/L ZnO NPs yielded the highest biomass, with 14.176 g fresh weight (FW) and 2.476 g dry weight (DW). The highest 2M4VP production was also observed at this concentration of ZnO NPs, reaching 7.84 mg/g DW, representing an 8.17-fold increase. ZnO NPs also significantly enhanced antioxidant activity, with increases of 1.36-fold in DPPH, 1.92-fold in HRSA, and 1.03-fold in ABTS+ compared to the controls. Applying ZnO NPs in plant tissue culture presents a promising strategy for enhancing 2M4VP production and antioxidant activity in C. grandis, underscoring their potential for pharmaceutical applications.