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<p>The purpose of this study is to investigate the methanol extract and the identified constituents of <i>Gossypium barbadense</i> leaves' antidiabetic and antioxidant properties. The capacity of <i>G. barbadense</i> leaf methanol extract (GBME) to scavenge DPPH free radicals, lower ferric iron, and act as an iron chelator was assessed. FeSO<sub>4</sub>-caused oxidative pancreatic damage was treated with varying GBME doses. Metformin was used as a reference to determine the methanol extract's inhibitory effect of α-amylase and α-glucosidase enzyme activities. After molecular docking of the reported constituents of <i>G. barbadense</i> against α-amylase (pdb id: 1hny) and α-glucosidase (pdb id: 3wy1), ADMET analysis was performed on the two top constituents. The antioxidant properties of <i>G. barbadense</i> increase significantly (p &lt; 0.05) with concentration compared to standard quercetin. The activities of ENTPDase, catalase, and malondialdehyde were reduced in FeSO<sub>4</sub>-injured pancreas when treated with methanol extract. Similar to the pattern seen with the common medication metformin, the methanol extract demonstrated a dose-dependent inhibitory capacity against the α-amylase and α-glucosidase enzymes. Additionally, the FeSO<sub>4</sub>-injured pancreas had a high amount of ATPase after treatment with methanol extract. The two constituents that demonstrated the strongest inhibitory effects on the α-amylase and α-glucosidase enzymes in silico were quercetin-3-O-β-glucoside and quercetin-3-O-(6-acetyl-β-glucoside). The computed values for human intestinal absorption and the brain barrier from ADMET analysis fell within the same range as the reference chemical (metformin). Consequently, <i>G. barbadense</i> leaves may represent a novel approach to diabetes treatment.</p>

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Antioxidant and antidiabetic activities of Gossypium barbadense L. leaves using in vitro, and in silico methods

  • 期刊论文

  • 发表日期：

    2025年5月15日

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  • Adesoji Alani Olanrewaju,
  • Akingbolabo Daniel Ogunlakin,
  • Deborah Olayinka Ogundele,
  • Abel Kolawole Oyebamiji,
  • Oluwafemi Adeleke Ojo,
  • Omowumi Temitayo Akinola,
  • Olayemi Elizabeth Arigbede,
  • David Gbenga Oke

摘要

The purpose of this study is to investigate the methanol extract and the identified constituents of Gossypium barbadense leaves' antidiabetic and antioxidant properties. The capacity of G. barbadense leaf methanol extract (GBME) to scavenge DPPH free radicals, lower ferric iron, and act as an iron chelator was assessed. FeSO4-caused oxidative pancreatic damage was treated with varying GBME doses. Metformin was used as a reference to determine the methanol extract's inhibitory effect of α-amylase and α-glucosidase enzyme activities. After molecular docking of the reported constituents of G. barbadense against α-amylase (pdb id: 1hny) and α-glucosidase (pdb id: 3wy1), ADMET analysis was performed on the two top constituents. The antioxidant properties of G. barbadense increase significantly (p < 0.05) with concentration compared to standard quercetin. The activities of ENTPDase, catalase, and malondialdehyde were reduced in FeSO4-injured pancreas when treated with methanol extract. Similar to the pattern seen with the common medication metformin, the methanol extract demonstrated a dose-dependent inhibitory capacity against the α-amylase and α-glucosidase enzymes. Additionally, the FeSO4-injured pancreas had a high amount of ATPase after treatment with methanol extract. The two constituents that demonstrated the strongest inhibitory effects on the α-amylase and α-glucosidase enzymes in silico were quercetin-3-O-β-glucoside and quercetin-3-O-(6-acetyl-β-glucoside). The computed values for human intestinal absorption and the brain barrier from ADMET analysis fell within the same range as the reference chemical (metformin). Consequently, G. barbadense leaves may represent a novel approach to diabetes treatment.