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<p>Fusarium wilt, caused by <i>Fusarium oxysporum</i> f.sp. <i>cubense</i> (<i>Foc</i>), poses a significant threat to banana crops. Circular RNAs (circRNAs), a newly discovered group of non-coding RNA, play a role in gene regulation. However, <i>Musa</i> spp. has not been explored for circRNA detection. This study presents the first identification of circRNAs in resistant and susceptible banana cultivars infected with <i>Foc</i> race 1 and TR4. A substantial number of circRNAs were identified from RNA-seq libraries of infected corm and root tissues. Differential expression analysis revealed specific circRNAs associated with <i>Foc</i>-resistant and susceptible cultivars. Functional annotation and pathway analysis highlighted involvement in secondary metabolite biosynthesis, Mitogen-activated protein kinases (MAPK) signalling pathway, and plant-pathogen interaction. Utilizing the differentially expressed circRNAs, we conducted a microRNA miRNA-mediated interaction analysis to identify potential sponge interactions. Subsequently, a comprehensive circRNA–miRNA–messenger RNA (mRNA) network was constructed, incorporating target genes directly or indirectly associated with plant resistance. This network sheds light on the intricate regulatory mechanisms underlying plant resistance to Fusarium wilt and provides valuable insights into the complex interplay between circRNAs, miRNAs, and mRNAs in banana defense responses.</p>

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Transcriptome-wide identification and characterization of Musa circular RNAs in corm and root tissues of resistant and susceptible cultivars in response to Fusarium oxysporum f.sp. cubense race1 and TR4

  • 期刊论文

  • 发表日期：

    2025年5月27日

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  • Chandrasekar Arumugam,
  • Anuradha Chelliah,
  • Prashina Mol Punchakkara,
  • Backiyarani Suthanthiram,
  • Thangavelu Raman,
  • Selvarajan Ramasamy

摘要

Fusarium wilt, caused by Fusarium oxysporum f.sp. cubense (Foc), poses a significant threat to banana crops. Circular RNAs (circRNAs), a newly discovered group of non-coding RNA, play a role in gene regulation. However, Musa spp. has not been explored for circRNA detection. This study presents the first identification of circRNAs in resistant and susceptible banana cultivars infected with Foc race 1 and TR4. A substantial number of circRNAs were identified from RNA-seq libraries of infected corm and root tissues. Differential expression analysis revealed specific circRNAs associated with Foc-resistant and susceptible cultivars. Functional annotation and pathway analysis highlighted involvement in secondary metabolite biosynthesis, Mitogen-activated protein kinases (MAPK) signalling pathway, and plant-pathogen interaction. Utilizing the differentially expressed circRNAs, we conducted a microRNA miRNA-mediated interaction analysis to identify potential sponge interactions. Subsequently, a comprehensive circRNA–miRNA–messenger RNA (mRNA) network was constructed, incorporating target genes directly or indirectly associated with plant resistance. This network sheds light on the intricate regulatory mechanisms underlying plant resistance to Fusarium wilt and provides valuable insights into the complex interplay between circRNAs, miRNAs, and mRNAs in banana defense responses.