<p>Organogenesis is a reliable method for the commercial micropropagation of date palm cultivars, contributing to the preservation of the date palm heritage in oasis regions. However, the persistence of endophytic bacterial contamination (EBC), often exacerbated by mechanisms such as sporulation, limits the efficiency of this promising technique. This comprehensive review examines the current state of knowledge on endophytic bacteria in in vitro date palm cultivation. It analyzes the factors promoting contamination, addresses the challenges posed by the sporulation mechanism, outlines methods for detecting and identifying bacterial contaminants, and highlights current control strategies. Furthermore, we emphasize the critical importance of adhering to good laboratory practices and the urgent need for further research to better understand the mechanisms of bacterial contamination. By exploring the complexities of date palm micropropagation, this study reveals the underlying challenges of bacterial contamination and underscores the resolution of these issues as essential for the sustainability of the date palm industry.</p>

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In vitro culture of date palm: a review of challenges and solutions for managing endophytic bacteria contamination

  • Mohamed Laaguidi,
  • Reda Meziani,
  • Khalid Sellam

摘要

Organogenesis is a reliable method for the commercial micropropagation of date palm cultivars, contributing to the preservation of the date palm heritage in oasis regions. However, the persistence of endophytic bacterial contamination (EBC), often exacerbated by mechanisms such as sporulation, limits the efficiency of this promising technique. This comprehensive review examines the current state of knowledge on endophytic bacteria in in vitro date palm cultivation. It analyzes the factors promoting contamination, addresses the challenges posed by the sporulation mechanism, outlines methods for detecting and identifying bacterial contaminants, and highlights current control strategies. Furthermore, we emphasize the critical importance of adhering to good laboratory practices and the urgent need for further research to better understand the mechanisms of bacterial contamination. By exploring the complexities of date palm micropropagation, this study reveals the underlying challenges of bacterial contamination and underscores the resolution of these issues as essential for the sustainability of the date palm industry.