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<p>In contemporary agriculture, pesticides such as organophosphates are frequently employed to boost crop yields and safeguard plants. However, they have negative effects on the ecosystem such as contaminated soil, nitrate leaching, decreased fertility, increased resistance to flora and fauna, and contaminated surface and groundwater. Chlorpyrifos [O,O-diethyl O-(3,5,6-trichlor- 2-pyridyl) phosphonothioate] is an organophosphate insecticide which is used extensively to control insects. An environmentally safe way to get rid of extra chlorpyriphos in soil and water can be microbial breakdown of pesticides. Effective strains for decomposing chlorpyriphos have been hard to isolate, with few cases documented, despite its extensive use since 1965. For our current study, six bacterial species were identified by isolating them from heavily pesticide-contaminated locations of the National Capital Region in and around Delhi. Further, they were assessed on nutrient- and enrichment-media for their degradation ability of chlorpyriphos at concentrations of 0.1%, 1%, and 10% by analytical techniques like thin layer chromatography (TLC) and gas chromatography (GC). As a result, TLC demonstrated complete degradation on the 10th day, however, the GC study showed different degradation levels of the Chlorpyrifos in nutritional media. These results demonstrate the future implication of these strains for bioremediation of habitats contaminated with chlorpyriphos.</p>

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Isolation, identification and characterization of microbes with a potential for organophosphate bioremediation

  • 期刊论文

  • 发表日期：

    2025年4月16日

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  • Abhinav Datta,
  • Tripti Bhatnagar,
  • Dipak Kumar Sahu,
  • Somya Agarwal,
  • Padmalaya Das

摘要

In contemporary agriculture, pesticides such as organophosphates are frequently employed to boost crop yields and safeguard plants. However, they have negative effects on the ecosystem such as contaminated soil, nitrate leaching, decreased fertility, increased resistance to flora and fauna, and contaminated surface and groundwater. Chlorpyrifos [O,O-diethyl O-(3,5,6-trichlor- 2-pyridyl) phosphonothioate] is an organophosphate insecticide which is used extensively to control insects. An environmentally safe way to get rid of extra chlorpyriphos in soil and water can be microbial breakdown of pesticides. Effective strains for decomposing chlorpyriphos have been hard to isolate, with few cases documented, despite its extensive use since 1965. For our current study, six bacterial species were identified by isolating them from heavily pesticide-contaminated locations of the National Capital Region in and around Delhi. Further, they were assessed on nutrient- and enrichment-media for their degradation ability of chlorpyriphos at concentrations of 0.1%, 1%, and 10% by analytical techniques like thin layer chromatography (TLC) and gas chromatography (GC). As a result, TLC demonstrated complete degradation on the 10th day, however, the GC study showed different degradation levels of the Chlorpyrifos in nutritional media. These results demonstrate the future implication of these strains for bioremediation of habitats contaminated with chlorpyriphos.