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<p><i>Ipomea purpurea</i> (L.) Roth. commonly known as Morning Glory is widely recognised as commercial, ornamental, and medicinal plant for its ethnomedicinal properties. This plant has been used in traditional medicines to cure a variety of ailments throughout the world. An effective and reproducible plant regeneration method has been developed for <i>I. purpurea</i> from in vitro explants. Callus has been induced from 78.33% internode explants excised from one-week old seedlings on Murashige and Skoog (MS) medium fortified with 2,4-dichlorophenoxyacetic acid (2,4-D) at 1.5&#xa0;mg/l. Callus proliferation was achieved using a combination of 1.5&#xa0;mg/l 2,4-D and 0.5&#xa0;mg/l NAA from internode explants. Adventitious shoots were successfully regenerated from callus on MS medium with 1.0&#xa0;mg/l thidiazuron (TDZ). ​ Shoot proliferation was achieved with 1.0&#xa0;mg/l TDZ and 0.5&#xa0;mg/l NAA, showing a 98% response. ​ The regenerated shoots developed roots on half-strength MS medium with 0.5&#xa0;mg/l indole-3-butyric acid (IBA). ​ The rooted plantlets were then transferred to a peat, vermiculite, and perlite mix (1:1:1) and had an over 85% survival rate within a month. ​ This method can produce hundreds of plants and support genetic studies in <i>Ipomoea</i> species.</p>

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In vitro regeneration of Ipomoea purpurea (L.) Roth via indirect organogenesis from seedling-derived internode explants

  • 期刊论文

  • 发表日期：

    2025年4月9日

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  • Rengasamy Anbazhakan,
  • Neng-Qi- Li,
  • Brihaspati Poudel,
  • Jiang-Yun- Gao

摘要

Ipomea purpurea (L.) Roth. commonly known as Morning Glory is widely recognised as commercial, ornamental, and medicinal plant for its ethnomedicinal properties. This plant has been used in traditional medicines to cure a variety of ailments throughout the world. An effective and reproducible plant regeneration method has been developed for I. purpurea from in vitro explants. Callus has been induced from 78.33% internode explants excised from one-week old seedlings on Murashige and Skoog (MS) medium fortified with 2,4-dichlorophenoxyacetic acid (2,4-D) at 1.5 mg/l. Callus proliferation was achieved using a combination of 1.5 mg/l 2,4-D and 0.5 mg/l NAA from internode explants. Adventitious shoots were successfully regenerated from callus on MS medium with 1.0 mg/l thidiazuron (TDZ). ​ Shoot proliferation was achieved with 1.0 mg/l TDZ and 0.5 mg/l NAA, showing a 98% response. ​ The regenerated shoots developed roots on half-strength MS medium with 0.5 mg/l indole-3-butyric acid (IBA). ​ The rooted plantlets were then transferred to a peat, vermiculite, and perlite mix (1:1:1) and had an over 85% survival rate within a month. ​ This method can produce hundreds of plants and support genetic studies in Ipomoea species.