The performance of FTIR and UV-Vis spectroscopy combined with chemometrics to classify Impatiens balsamina L. leaf extracts from different solvent extraction
摘要
Impatiens balsamina L. is a home garden plant that has often been utilized by the community as a traditional medicine for painkillers and menstrual cramps. A previous study has successfully utilized this plant as an antimicrobial, antirheumatic, antipruritic, antiinflammation, anti-allergic, and antitumor agent. The bioactive compounds and the bioactivity of medicinal plants depend on several factors, like different solvent extraction. The combination of FTIR and UV-Vis spectra with chemometric technique becomes a choice to classify the I. balsamina leaf extract from different solvent extraction. Extraction was performed using a sonication method with the yield obtained from methanolic, ethyl-acetic, and chloroform extract were 31.93% ± 1.975, 16.20% ± 1.651, and 16.71% ± 1.742, respectively. The FTIR spectrum was measured at 600–4000 cm− 1 wavenumbers, while the UV-Vis spectrum was measured at 200–280 nm wavelengths. The I. balsamina leaf extracts obtained a similar absorbance value in FTIR for ethyl-acetic and chloroform extracts, but different from methanolic extract. The PCA grouping was performed for further classification assisted by orange data mining software. The total value of PC from UV-Vis spectra was 90% and the total value of PC from FTIR spectra was 85%. Therefore, the I. balsamina leaf extract can be grouped based on different solvent extractions. A confusion matrix analysis presents few samples of ethyl-acetic extract are described as chloroform extract, and vice versa, following the PCA results from either FTIR or UV-Vis spectra.