Decontamination of nodal segments from four native fruit species from Brazil related to sonication, asepsis time, and the use of antibiotics
摘要
Myrtaceae is one of Brazil’s most significant plant families, including fruit trees and shrubs with a crucial role in the maintenance of the ecosystem. Although many Myrtaceous have agronomic potential, the production of nursery trees faces challenges due to seed recalcitrancy and low success on traditional vegetative multiplication methods. Micropropagation is a promising alternative, however, high contamination rates pose a significant barrier. This study evaluated the effectiveness of sonication, asepsis time, and the use of antibiotics in the decontamination of nodal segments from four native Myrtaceae fruit tree species: Campomanesia phaea, Eugenia brasiliensis, E. involucrata, and E. pyriformis. Sonication combined with a 20-minute asepsis treatment in NaOCl efficiently enhanced the control of fungal contamination, with no negative impact on oxidation and sprout emergence. Bacterial contamination was best controlled through the supplementation with antibiotics to the culture medium, with 500 mg L⁻¹ cefotaxime and a combination of 250 mg L⁻¹ cefotaxime and 200 mg L⁻¹ Timentin® being the most effective treatments.