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<p>Plant-derived bioactive compounds have gained great importance in several sectors, like pharmaceuticals, cosmetics, food ,and agriculture, as an alternative to synthetic antibiotics for the&#xa0;prevention and treatment of infectious diseases, additives, flavouring agents, and botanical pesticides. The present study was aimed at evaluating&#xa0; phytochemical components, antibacterial activity, and identifying the bioactive compounds present in the leaf extracts of <i>Rubus ellipticus</i>. Four different solvents were employed in the extraction process. The antibacterial activity was carried out through the&#xa0;agar well diffusion method against four pathogens, namely, <i>Acinetobacter baumannii</i> (MTCC 1145), <i>Staphylococcus aureus</i> (MTCC 7443), <i>Pseudomonas aeruginosa</i> (MTCC 1145), and <i>Bacillus subtilis</i> (MTCC 736). Further, the phytochemical constituents, such as, carbohydrates, flavonoids, terpenoids, and alkaloids were evaluated. The ethanolic extract of <i>R</i>. <i>ellipticus</i> leaves (ERE) exhibited maximum antibacterial activity against the selected pathogens by&#xa0;showing a&#xa0;15&#xa0;mm inhibition zone against <i>S</i>. <i>aureus</i> and <i>P</i>. <i>aeruginosa</i>. The size of the inhibition zone showed by ERE was 13.3&#xa0;mm and 12.6&#xa0;mm against <i>B</i>. <i>subtilis</i> and <i>A</i>. <i>baumannii</i>, respectively. Hence, ERE was picked for the thin layer chromatography using toluene and ethyl acetate (93:7) as the&#xa0;mobile phase. Following plate development, four bands (ETE1, ETE2, ETE3, and ETE4) were discerned and subsequently assessed for antibacterial activity. ETE3 fraction exhibited maximum inhibitory activity against the selected bacterial strains, and therefore, it was further screened for FT–IR and GC–MS analysis. Pyridine was identified as the principal molecule (peak area 77.12%) responsible for the inhibitory action of the extract. Pyridine consists of a heterocyclic ring with a&#xa0;C<sub>5</sub>H<sub>5</sub>N chemical formula and is&#xa0;made up of alkaloids. Results of this study confer the utility of leaf extracts of <i>R</i>. <i>ellipticus</i> to develop new and effective&#xa0;alternative to combat the implications of drug resistance, as the bioactive compounds present in the leaf extract&#xa0;have shown effective antibacterial activity against the selected pathogens responsible for nosocomial and community-acquired infection.</p>

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Phytochemical composition, antibacterial activity, and metabolomic analysis of Rubus elipticus Sm. leaf extracts

  • 期刊论文

  • 发表日期：

    2025年3月14日

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  • Vipin Nautiyal,
  • Sweta Kumari

摘要

Plant-derived bioactive compounds have gained great importance in several sectors, like pharmaceuticals, cosmetics, food ,and agriculture, as an alternative to synthetic antibiotics for the prevention and treatment of infectious diseases, additives, flavouring agents, and botanical pesticides. The present study was aimed at evaluating  phytochemical components, antibacterial activity, and identifying the bioactive compounds present in the leaf extracts of Rubus ellipticus. Four different solvents were employed in the extraction process. The antibacterial activity was carried out through the agar well diffusion method against four pathogens, namely, Acinetobacter baumannii (MTCC 1145), Staphylococcus aureus (MTCC 7443), Pseudomonas aeruginosa (MTCC 1145), and Bacillus subtilis (MTCC 736). Further, the phytochemical constituents, such as, carbohydrates, flavonoids, terpenoids, and alkaloids were evaluated. The ethanolic extract of R. ellipticus leaves (ERE) exhibited maximum antibacterial activity against the selected pathogens by showing a 15 mm inhibition zone against S. aureus and P. aeruginosa. The size of the inhibition zone showed by ERE was 13.3 mm and 12.6 mm against B. subtilis and A. baumannii, respectively. Hence, ERE was picked for the thin layer chromatography using toluene and ethyl acetate (93:7) as the mobile phase. Following plate development, four bands (ETE1, ETE2, ETE3, and ETE4) were discerned and subsequently assessed for antibacterial activity. ETE3 fraction exhibited maximum inhibitory activity against the selected bacterial strains, and therefore, it was further screened for FT–IR and GC–MS analysis. Pyridine was identified as the principal molecule (peak area 77.12%) responsible for the inhibitory action of the extract. Pyridine consists of a heterocyclic ring with a C5H5N chemical formula and is made up of alkaloids. Results of this study confer the utility of leaf extracts of R. ellipticus to develop new and effective alternative to combat the implications of drug resistance, as the bioactive compounds present in the leaf extract have shown effective antibacterial activity against the selected pathogens responsible for nosocomial and community-acquired infection.