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<p><i>Buchanania cochinchinensis</i> (Lour.) Almeda, a socioeconomically important tree in the Anacardiaceae family, is valued for its versatile use, afforestation potential and medicinal value. Despite its socioeconomic significance, molecular studies on this species using microsatellite markers are lacking. The present study optimized a high-throughput DNA extraction protocol for fresh and stored leaf samples, addressing the challenges posed by secondary metabolites in such forest trees. The isolated DNA was checked via UV Spectrophotometry, which revealed A260/A280 ratios ranging from 1.65 to 1.83, indicating the suitability of the isolated DNA for polymerase chain reaction (PCR) amplification. Owing to the unavailability of specific microsatellite primers for <i>B. cochinchinensis</i>, a cross-species transferability approach was employed using microsatellite markers from other species, i.e., <i>Cotinus coggygria</i> and <i>Mangifera indica</i> of the Anacardiaceae family. Among the fourteen SSR primers screened, four exhibited cross-species transferability and were further characterized. Marker characteristics such as heterozygosity (mean H: 0.42), polymorphic information content (mean PIC: 0.33), effective multiplex ratio (mean: 1.0), discrimination power (mean DP: 0.71), and resolving power (mean RP: 0.92) were evaluated. The gene diversity (mean = 0.38) and Shannon information index (mean = 0.56) were also estimated. These findings indicate that the cross-transferred microsatellite markers are effective for genetic studies in <i>B. cochinchinensis</i>. These markers can be utilized for further molecular characterization and genetic diversity assessment of this economically important species.</p>

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Enhancing conservation and genetic resource management through cross-transferability exploration and optimization of DNA extraction protocol for Buchanania cochinchinensis (Lour.) M. R. Almeida

  • 期刊论文

  • 发表日期：

    2025年1月29日

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  • Ankur Dahayat,
  • Naseer Mohammad,
  • Raj Singh Yadav,
  • Harshita Agrahari,
  • Yogesh Pardhi,
  • Fatima Shirin,
  • Bilal Miskini

摘要

Buchanania cochinchinensis (Lour.) Almeda, a socioeconomically important tree in the Anacardiaceae family, is valued for its versatile use, afforestation potential and medicinal value. Despite its socioeconomic significance, molecular studies on this species using microsatellite markers are lacking. The present study optimized a high-throughput DNA extraction protocol for fresh and stored leaf samples, addressing the challenges posed by secondary metabolites in such forest trees. The isolated DNA was checked via UV Spectrophotometry, which revealed A260/A280 ratios ranging from 1.65 to 1.83, indicating the suitability of the isolated DNA for polymerase chain reaction (PCR) amplification. Owing to the unavailability of specific microsatellite primers for B. cochinchinensis, a cross-species transferability approach was employed using microsatellite markers from other species, i.e., Cotinus coggygria and Mangifera indica of the Anacardiaceae family. Among the fourteen SSR primers screened, four exhibited cross-species transferability and were further characterized. Marker characteristics such as heterozygosity (mean H: 0.42), polymorphic information content (mean PIC: 0.33), effective multiplex ratio (mean: 1.0), discrimination power (mean DP: 0.71), and resolving power (mean RP: 0.92) were evaluated. The gene diversity (mean = 0.38) and Shannon information index (mean = 0.56) were also estimated. These findings indicate that the cross-transferred microsatellite markers are effective for genetic studies in B. cochinchinensis. These markers can be utilized for further molecular characterization and genetic diversity assessment of this economically important species.