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<p>In this study, we developed a protocol to induce PLBs of <i>Prosthechea fragans</i> (Sw.) W.E. Higgins through in vitro culture. For this purpose, three-month-old <i>P. fragans</i> protocorms were used and inoculated on Murashige &amp; Skoog (MS) basal medium with 500 mg L<sup>− 1</sup> of hydrolyzed casein (CH) and different Zeatin (Z) concentrations (0, 0.25, 0.40, 0.50, 0.60, and 0.75 mg L<sup>− 1</sup>). In vitro, cultured protocorms were exposed to two light conditions: fluorescent light and red LED light. The results showed that protocorms grown on MS medium supplemented with 0.50 mg L<sup>− 1</sup> Zeatin formed of 101 ± 0.37 PLBs in the 7th week of culture under red LED light. Furthermore, this is the first report of PLBs induction in <i>P. fragans</i> using Zeatin, as a plant growth regulator, from the use of protocorms.</p>

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Effect of lighting spectrums in the induction of PLBs in Prosthechea fragans (Sw.) W.E. Higgins (Orchidaceae)

  • 期刊论文

  • 发表日期：

    2024年12月31日

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  • Brandon Giovanni Cervantes-Rodríguez,
  • Lourdes Georgina Iglesias-Andreu

摘要

In this study, we developed a protocol to induce PLBs of Prosthechea fragans (Sw.) W.E. Higgins through in vitro culture. For this purpose, three-month-old P. fragans protocorms were used and inoculated on Murashige & Skoog (MS) basal medium with 500 mg L− 1 of hydrolyzed casein (CH) and different Zeatin (Z) concentrations (0, 0.25, 0.40, 0.50, 0.60, and 0.75 mg L− 1). In vitro, cultured protocorms were exposed to two light conditions: fluorescent light and red LED light. The results showed that protocorms grown on MS medium supplemented with 0.50 mg L− 1 Zeatin formed of 101 ± 0.37 PLBs in the 7th week of culture under red LED light. Furthermore, this is the first report of PLBs induction in P. fragans using Zeatin, as a plant growth regulator, from the use of protocorms.