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<p>The present study aimed to standardize a cormel in vitro propagation protocol for <i>Gladiolus hybridus</i> Hort<i>.</i> cv 'Red Majesty'. The study evaluated many parameters, from culture establishment to cormlet development of <i>Gladiolus hybridus</i> Hort<i>.</i> There was a significant effect of treatments on these parameters and a 3-step process was standardized for its commercial micropropagation. Minimum (45) days to cormlet appearance was recorded on MS + B<sub>5</sub> vitamins supplemented with CCC (1mgl<sup>−1</sup>) + sucrose (9%), while the maximum average number of cormels per explant (14) was produced on MS + B<sub>5</sub> vitamins supplemented with IBA (0.5 mgl<sup>−1</sup>) + CCC (2 mgl<sup>−1</sup>) + sucrose (3%). Maximum cormel development percent (85) was observed on MS + B5 vitamins supplemented with growth regulator combination CCC (1.0mgl<sup>−1</sup>) + sucrose (9%). Maximum number of cormels (14) was observed in (IBA 0.5mgl<sup>−1</sup> + CCC 2mgl<sup>−1</sup> sucrose 3%). Overall the best treatment for&#xa0;the combined effect on cormlet development (w.r.t. cormlet maturation percentage and the number of cormels) was MS + B<sub>5</sub> medium with IBA (0.5&#xa0;mg<sup>−1</sup>) + CCC (2&#xa0;mg<sup>−1</sup>) + sucrose (6%) as 12 number of cormels per explant and 85 cormlet development percent was achieved. In 150&#xa0;days, 12–14 cormels were obtained from a single corm slice, and each cormel size was approximately 22&#xa0;mm. Therefore, the multiplication rate of superior gladiolus hybrid 'Red Majesty' can be increased 14-fold while reducing the multiplication duration using the in vitro protocol developed in the present study.</p>

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A high-efficiency 3-Step in vitro protocol for commercial cormel micropropagation of Gladiolus hybridus Hort. cv ‘Red Majesty’

  • 期刊论文

  • 发表日期：

    2024年12月31日

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  • Madeeha Naik,
  • Imtiyaz T. Nazki,
  • Amjad M. Husaini

摘要

The present study aimed to standardize a cormel in vitro propagation protocol for Gladiolus hybridus Hort. cv 'Red Majesty'. The study evaluated many parameters, from culture establishment to cormlet development of Gladiolus hybridus Hort. There was a significant effect of treatments on these parameters and a 3-step process was standardized for its commercial micropropagation. Minimum (45) days to cormlet appearance was recorded on MS + B5 vitamins supplemented with CCC (1mgl−1) + sucrose (9%), while the maximum average number of cormels per explant (14) was produced on MS + B5 vitamins supplemented with IBA (0.5 mgl−1) + CCC (2 mgl−1) + sucrose (3%). Maximum cormel development percent (85) was observed on MS + B5 vitamins supplemented with growth regulator combination CCC (1.0mgl−1) + sucrose (9%). Maximum number of cormels (14) was observed in (IBA 0.5mgl−1 + CCC 2mgl−1 sucrose 3%). Overall the best treatment for the combined effect on cormlet development (w.r.t. cormlet maturation percentage and the number of cormels) was MS + B5 medium with IBA (0.5 mg−1) + CCC (2 mg−1) + sucrose (6%) as 12 number of cormels per explant and 85 cormlet development percent was achieved. In 150 days, 12–14 cormels were obtained from a single corm slice, and each cormel size was approximately 22 mm. Therefore, the multiplication rate of superior gladiolus hybrid 'Red Majesty' can be increased 14-fold while reducing the multiplication duration using the in vitro protocol developed in the present study.