In vitro germination of immature seeds under two lighting spectrums to obtain protocorms in Vanilla planifolia Jacks.

In the present study, cross pollination and self-pollination schemes were carried out to obtain seeds in Vanilla planifolia Jacks. Differences in average lengths at 45 days after pollination (DAP) were found to be 17.72 ± 0.65 and 16.22 ± 0.44 cm in fruits from cross pollination and self-pollination, respectively, while for average fruit weight was 13.5 ± 1.24 and 11.25 ± 0.62 g in cross pollination and self-pollination, respectively. Seeds were inoculated on Murashige & Skoog (MS) medium at half concentration supplemented with 15 gL^− 1 sucrose and 500 mgL^− 1 in hydrolyzed casein. The cultures were maintained under two illumination conditions: fluorescent light and red LED. The efficiency of red LED light for germination of V. planifolia seeds in vitro, obtained by self-pollination and cross pollination, and for both sucrose concentrations of 15 gL^− 1 and 30 gL^− 1 evaluated, was verified. It was found that the most effective treatment was that of 15 gL^− 1 in seeds from cross pollination with 90 ± 1.29% in the in vitro germination, which contrasted with the lowest values obtained for in vitro germination obtained in seeds from self-pollination 8.33 ± 2.39% grown with a sucrose concentration of 15 gL^− 1.