“In vitro callus generation and somatic embryogenesis from leaf explant of Madhuca longifolia var latifolia (Roxb.) A. Chev”

The study, “In vitro Callus Induction and Somatic Embryogenesis from Leaf Explants of Madhuca longifolia var. latifolia,” was conducted at Tissue Culture Laboratory, Forest College and Research Institute, Mulugu, Siddipet. A protocol was established for in vitro propagation and indirect organogenesis using leaf explants of Madhuca longifolia. Highest callus induction was achieved with Woody Plant Medium (WPM) containing 2 mg/L thidiazuron (TDZ), while Murashige and Skoog (MS) medium with 6 mg/L benzylaminopurine (BAP) and 0.5 mg/L naphthaleneacetic acid (NAA) effectively promoted somatic embryogenesis. These findings provide a foundation for the development of advanced propagation techniques for Madhuca longifolia, offering significant potential for its conservation and sustainable management. This study also explores the generation of synthetic seeds from somatic embryos, capable of developing into complete plants under both in-vitro and ex-vitro conditions, even in unseasonal environments. This established protocol presents a scalable strategy for the production and extraction of bioactive compounds, which is crucial for pharmaceutical and nutraceutical applications. Furthermore, this work lays the groundwork for future research on genetic improvement, abiotic stress tolerance, and biosynthesis of secondary metabolites in Madhuca longifolia.