<p>Entanglement detection, the process of verifying quantum entanglement is a fundamental challenge in quantum information processing. Various approaches have been proposed to address this challenge, with many recent studies applying supervised machine learning methods. While these methods have demonstrated high accuracy in entanglement detection, it is reasonable to assume that the entangled states themselves are not definitively known. To address this limitation, we have devised a machine learning method for entanglement detection based on positive-unlabeled learning, a classical machine learning framework that does not use label information from negative data. Using a deep neural network model to synthetic dataset under the assumption of mixed states, we conducted experiments on a classical computer to valid the effectiveness and characteristics of the proposed method. Our approach introduces a novel framework that accounts for the data generation constraints in the training process of entanglement detector, thereby advancing machine learning techniques in quantum information science.</p>