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<p>Fostering nano formulations has been recognized as&#xa0;top priority in treating inflammatory diseases. In our present work, metal oxide nanoparticles synthesis was prepared using methanol extract of&#xa0;<i>Quercus infectoria</i> Olivier. Phytoconstituents of the methanol extract were analyzed which showed the presence of secondary metabolites viz., flavonoids, alkaloids, tannins, phenols, proteins and steroids. The biosynthesized zinc oxide nanoparticles were characterized for their optical property, particle size, zeta potential, FT-IR, X-ray diffraction and scanning electron microscope, further investigated for its&#xa0;antioxidant, anti-inflammatory and cytotoxicity through in vitro&#xa0;techniques. The appearance a peak at 354&#xa0;nm in optical property confirms the surface plasma resonance of zinc oxide nanoparticle formation. Particle size and zeta potential were observed to be 95.54&#xa0;nm and − 13.6&#xa0;mV respectively indicating the particles were in the nanoscale with good stability. The FT-IR studies outlined the existence of several functional groups in the extract. The SEM analysis depicted its structure to be spongy and XRD analysis peaks at 2θ values ranging between 31.73° and 76.90° indicated the purity of zinc oxide nanoparticles. The DPPH radical scavenging study showed 89.87 ± 0.57% of inhibition at 500&#xa0;μg/mL indicating its Antioxidant activity. The anti-inflammatory activity study was investigated by anti-proteinase and protein denaturation assay methods which exhibited 73.50 ± 0.36% and 79.12 ± 0.67% of inhibition at 500&#xa0;μg/mL indicated its good anti-inflammatory activity. The zinc oxide nanoparticles demonstrated anti-inflammatory action on RAW264.7 cell lines by reducing the pro-inflammatory mediators secretion triggered by lipopolysaccharide in Nitric oxide production assay. Our present research is the first endeavor in the biosynthesis of zinc oxide nanoparticles as an eco-friendly and economical from methanol extract of&#xa0;<i>Quercus infectoria</i> Olivier as an anti-inflammatory agent. Further, research into bio-guided fractions and its mechanism may bring up a strong foundation for a viable anti-inflammatory drug.</p>

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Macrophage-mediated anti-inflammatory enhancement by biosynthesized zinc oxide nanoparticles of Quercus infectoria Olivier extract in RAW 264.7 cells

  • 期刊论文

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  • 发表日期：

    2025年7月31日

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  • A. Umamaheswari,
  • S. Lakshmana Prabu,
  • K. Huthaya Prakash,
  • A. Puratchikody

摘要

Fostering nano formulations has been recognized as top priority in treating inflammatory diseases. In our present work, metal oxide nanoparticles synthesis was prepared using methanol extract of Quercus infectoria Olivier. Phytoconstituents of the methanol extract were analyzed which showed the presence of secondary metabolites viz., flavonoids, alkaloids, tannins, phenols, proteins and steroids. The biosynthesized zinc oxide nanoparticles were characterized for their optical property, particle size, zeta potential, FT-IR, X-ray diffraction and scanning electron microscope, further investigated for its antioxidant, anti-inflammatory and cytotoxicity through in vitro techniques. The appearance a peak at 354 nm in optical property confirms the surface plasma resonance of zinc oxide nanoparticle formation. Particle size and zeta potential were observed to be 95.54 nm and − 13.6 mV respectively indicating the particles were in the nanoscale with good stability. The FT-IR studies outlined the existence of several functional groups in the extract. The SEM analysis depicted its structure to be spongy and XRD analysis peaks at 2θ values ranging between 31.73° and 76.90° indicated the purity of zinc oxide nanoparticles. The DPPH radical scavenging study showed 89.87 ± 0.57% of inhibition at 500 μg/mL indicating its Antioxidant activity. The anti-inflammatory activity study was investigated by anti-proteinase and protein denaturation assay methods which exhibited 73.50 ± 0.36% and 79.12 ± 0.67% of inhibition at 500 μg/mL indicated its good anti-inflammatory activity. The zinc oxide nanoparticles demonstrated anti-inflammatory action on RAW264.7 cell lines by reducing the pro-inflammatory mediators secretion triggered by lipopolysaccharide in Nitric oxide production assay. Our present research is the first endeavor in the biosynthesis of zinc oxide nanoparticles as an eco-friendly and economical from methanol extract of Quercus infectoria Olivier as an anti-inflammatory agent. Further, research into bio-guided fractions and its mechanism may bring up a strong foundation for a viable anti-inflammatory drug.