<p>This study investigates the synthesis and characterization of borosilicate bioactive glass with the composition [50 B₂O₃–5 SiO₂–15 ZnO–30 CaO], focusing on the effects of gamma irradiation on its properties and drug delivery capabilities. The samples were prepared through the melt-quenching method and subjected to gamma radiation doses of 0.5, 1, and 2 mGy. Characterization techniques, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM/EDX), revealed that gamma irradiation increased the number of non-bridging oxygens in the glass network, enhancing its bioactivity while maintaining its amorphous structure. The cytotoxicity assay using Vero cells demonstrated improved biocompatibility with higher irradiation doses, with an IC₅₀ value of 463.21 μg/ml for the most irradiated sample. The encapsulation efficiency of ciprofloxacin (CFX) in borosilicate glass was confirmed, showing that all samples successfully loaded the drug, with the non-irradiated sample achieving the highest efficiency (53.65%). In vitro drug release studies indicated that the release profiles conformed to the Higuchi model, highlighting sustained release characteristics, particularly in samples irradiated with 2 mGy. The research suggests that gamma-irradiated borosilicate glass may be a promising biomaterial for drug delivery applications as antibiotics in treating bacterial bone infections, providing sustained antibiotic release over extended periods.</p>