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<p>An <i>in-situ</i> optimised electro-polymerised molecularly-imprinted polymer (MIP) on single-walled carbon nanotube-modified screen-printed carbon electrode (SWCNT/SPCE) embedded on a molecularly-imprinted poly(styrene-co–N-(3-aminopropyl)methacrylamide) (denoted as eMIP/SWCNT/SPCE) using the Box-Behnken Design (BBD) was able to reduce chemical consumption and process time. The <i>in-situ</i> optimisation steps resulted in a 1:25 template-monomer ratio, 19 cyclic voltammetry (CV) cycle number, and 250 mV/s of scan rate for 50&#xa0;µg/L of 25-hydroxyvitamin D<sub>3</sub> (25-OHD<sub>3</sub>), which was incubated for 10 min, prior wet laboratory work. The selectivity was controlled by comparing the response from MIP to non-imprinted polymer (NIP), producing an imprinting factor (IF) value of 3.1 at optimised condition. The calibration was plotted from 10.0 to 30.0&#xa0;µg/L of 25-OHD<sub>3</sub> with an limit of detection (LOD) of 1.8&#xa0;µg/L and limit of quantification (LOQ) of 5.5&#xa0;µg/L. The obtained 25-OHD<sub>3</sub> concentration in the serum samples of SRM978f was 12.9 ± 3.3&#xa0;µg/L (reference value 14.0 ± 4.6&#xa0;µg/L) and 10&#xa0;µg/L of spiked serum SRM909c was 10.3 ± 3.6&#xa0;µg/L. The modified sensor eMIP/SWCNT/SPCE was able to recover the real sample result from 92.1% to 103.0%.</p>

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Electropolymerised molecularly-imprinted polymer for the detection of serum Vitamin D using Box-Behnken design

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月31日

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  • Mohd Azerulazree Jamilan,
  • Zainiharyati Mohd Zain,
  • Kavirajaa Pandian Sambasevam,
  • Faizatul Shimal Mehamod,
  • Balqis Kamarudin,
  • Mohd Fairulnizal Md Noh

摘要

An in-situ optimised electro-polymerised molecularly-imprinted polymer (MIP) on single-walled carbon nanotube-modified screen-printed carbon electrode (SWCNT/SPCE) embedded on a molecularly-imprinted poly(styrene-co–N-(3-aminopropyl)methacrylamide) (denoted as eMIP/SWCNT/SPCE) using the Box-Behnken Design (BBD) was able to reduce chemical consumption and process time. The in-situ optimisation steps resulted in a 1:25 template-monomer ratio, 19 cyclic voltammetry (CV) cycle number, and 250 mV/s of scan rate for 50 µg/L of 25-hydroxyvitamin D3 (25-OHD3), which was incubated for 10 min, prior wet laboratory work. The selectivity was controlled by comparing the response from MIP to non-imprinted polymer (NIP), producing an imprinting factor (IF) value of 3.1 at optimised condition. The calibration was plotted from 10.0 to 30.0 µg/L of 25-OHD3 with an limit of detection (LOD) of 1.8 µg/L and limit of quantification (LOQ) of 5.5 µg/L. The obtained 25-OHD3 concentration in the serum samples of SRM978f was 12.9 ± 3.3 µg/L (reference value 14.0 ± 4.6 µg/L) and 10 µg/L of spiked serum SRM909c was 10.3 ± 3.6 µg/L. The modified sensor eMIP/SWCNT/SPCE was able to recover the real sample result from 92.1% to 103.0%.