Purpose <p>At present, Wheeled Mobile Loaders (WMLs), which are widely used in factories, have a complex structure and therefore dynamic stability studies are very important. This paper presents the dynamic stability analysis and optimization methods of WMLs for automation line of fish quick-freeze.</p> Method <p>A dynamical model of WMLs was established in Solidworks. Using ADAMS, the vibration character due to the collision force acting on the wheels was obtained according to the simulation of all the working processes and dynamic stability of the mechanical system was analyzed. Based on the simulation analysis, Analysis of Variance (ANOVA) was used to determine the optimal parameters to satisfy the stability of the mechanical system.</p> Results <p>The results show that dynamic analysis by collision force acting on wheels can be effectively used to improve the vibration characteristics of WMLs. It is also shown that dynamic stability simulation and design optimization for all the working processes can reduce vibration and eliminate the tendency of overturning.</p> Conclusion <p>The results show that ADAMS and ANOVA can actually be used to optimize or improve the quality of dynamic stability and design parameters. WMLs designed based on the optimal parameters satisfies the stability and this study can be helpful in the stability study of WMLs for automatic warehouse.</p>