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Background <p>Human platelet lysate (hPL) contains growth factors, cytokines, and proteins that support wound healing. However, preserving its bioactivity during storage remains challenging, especially for chronic wound treatment. This study evaluated the stability of lyophilized human platelet lysate (LhPL) and its combined therapeutic potential with Moringa oleifera extract.</p> Methods <p>Growth factors in hPL before and after lyophilization were quantified using ELISA to assess cytokine preservation. The antibacterial activity of Moringa oleifera crude extract against Pseudomonas aeruginosa and Staphylococcus aureus was evaluated using the well diffusion method. In vitro wound healing was assessed through cell migration assays using LhPL alone and in combination with Moringa oleifera extract.</p> Results <p>ELISA results showed that key cytokines and growth factors were preserved after lyophilization, improving the shelf life of hPL. Moringa oleifera extract demonstrated dose-dependent antibacterial activity against both bacterial strains. In vitro wound healing analysis showed 57.41% wound closure with LhPL alone, while the combination treatment achieved 67.21% wound closure, indicating enhanced cell migration and accelerated healing.</p> Conclusion <p>Lyophilized human platelet lysate retains its bioactivity after preservation and exhibits significant wound healing potential. Combining LhPL with Moringa oleifera extract further enhances antibacterial activity and wound healing efficiency, suggesting its potential application in the treatment of acute and chronic wounds, including diabetic ulcers, burn injuries, and surgical wounds.</p>

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Synergistic Effects of Lyophilized Human Platelet Lysate and Moringa Oleifera Leaf Extract on Fibroblast Migration and Antibacterial Activity: An In Vitro Study

  • 期刊论文

  • 发表日期：

    2026年5月23日

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  • Lakshmi Naathan M,
  • T. G Nithya,
  • Raghu Babu Pothireddy,
  • Seetha S Babu,
  • Angeline Julius,
  • Praveen Kumar L

摘要

Background

Human platelet lysate (hPL) contains growth factors, cytokines, and proteins that support wound healing. However, preserving its bioactivity during storage remains challenging, especially for chronic wound treatment. This study evaluated the stability of lyophilized human platelet lysate (LhPL) and its combined therapeutic potential with Moringa oleifera extract.

Methods

Growth factors in hPL before and after lyophilization were quantified using ELISA to assess cytokine preservation. The antibacterial activity of Moringa oleifera crude extract against Pseudomonas aeruginosa and Staphylococcus aureus was evaluated using the well diffusion method. In vitro wound healing was assessed through cell migration assays using LhPL alone and in combination with Moringa oleifera extract.

Results

ELISA results showed that key cytokines and growth factors were preserved after lyophilization, improving the shelf life of hPL. Moringa oleifera extract demonstrated dose-dependent antibacterial activity against both bacterial strains. In vitro wound healing analysis showed 57.41% wound closure with LhPL alone, while the combination treatment achieved 67.21% wound closure, indicating enhanced cell migration and accelerated healing.

Conclusion

Lyophilized human platelet lysate retains its bioactivity after preservation and exhibits significant wound healing potential. Combining LhPL with Moringa oleifera extract further enhances antibacterial activity and wound healing efficiency, suggesting its potential application in the treatment of acute and chronic wounds, including diabetic ulcers, burn injuries, and surgical wounds.